布洛芬对小鼠肺纤维化的保护作用

  目的 研究布洛芬对博莱霉素诱导的肺纤维化的治疗保护作用。方法 通过气管内滴注博莱霉素复制小鼠肺纤维化模型，实验分为正常对照组、模型组、布洛芬5 mg/kg治疗组、布洛芬10 mg/kg治疗组和布洛芬20 mg/kg治疗组。采集血清行一氧化氮（NO）化学法检测小鼠血清中NO含量；收集肺泡灌洗液行ELISA方法测定肿瘤坏死因子（TNF-α）和白细胞介素6（IL-6）含量；留取肺组织行HE染色观察各组小鼠肺组织形态变化，制备肺组织匀浆测定匀浆中丙二醛（MDA）含量；采用RT-PCR和Western blot方法检测肺组织中转化生长因子β1（TGF-β1）、Smad2和p-Smad2的表达水平。结果 与正常对照组比较，肺纤维化模型组肺组织中发生炎性细胞浸润、肺泡间隔增厚情况，同时血清中NO含量和肺泡灌洗液中炎性因子TNF-α和IL-6含量显著增加，小鼠肺组织中MDA含量也有所增加，TGF-β1和p-Smad2的表达显著升高。经布洛芬给药治疗后，各项生化指标有所下降，同时肺纤维化损伤得以缓解。结论 布洛芬可缓解由博莱霉素诱导的肺纤维化，其作用机制可能与下调NO含量、TNF-α和IL-6等炎性因子含量及调控TGF-β信号通路有关。

Experimental Study of Protective Effect of Ibuprofen on the Pulmonary Fibrosis in Mice

Objective To study the protective effect of ibuprofen on bleomycin-induced pulmonary fibrosis.Methods The pulmonary fibrosis mouse model was established by intratracheal instillation of bleomycin.The experimental mice were divided into normal control group,model group,5 mg/kg brufen treated group,10 mg/kg brufen treated group,and 20 mg/kg brufen treated group.Serum was collected to detect the content of NO by NO chemical method;lavage fluid was collected for determination of TNF-α and IL-6 levels by using ELISA;specimens of lung tissue were stained with HE to observe the morphological changes,and lung homogenates were prepared for determination of malondialdehyde(MDA);the expression levels TGF-β1,Smad2 and p-Smad2 in lung tissue were detected by RT-PCR and Western blot analysis.Results Compared with the normal control group,inflammatory cell infiltration,alveolar septal thickening occurred in lung tissue of pulmonary fibrosis model;meanwhile,NO content in serum and inflammatory cytokines TNF-α and IL-6 levels in bronchoalveolar lavage fluid were significantly increased,MDA content was also increased in the lung tissue of mice,the expressions of TGF-β1 and p-Smad2 were significantly increased;after ibuprofen administration,various biochemical indicators declined,and pulmonary fibrosis damage was mitigated.Conclusion Ibuprofen can alleviated bleomycin-induced pulmonary fibrosis,and the mechanism may be associated with lowered content of NO,TNF-α,IL-6 and other inflammatory cytokines content and regulation of TGF-β signaling pathway.