环孢素A联合脂多糖对牙周膜成纤维细胞生物学活性的影响

 目的 探讨环孢素A(CsA)联合牙龈卟啉单胞菌脂多糖(Pg-LPS)对人牙周膜成纤维细胞(PDLFs)生物学活性的影响。方法 将CsA（100 ng/mL）、LPS（100 ng/mL）分别单独及联合作用于人PDLFs，采用考马斯亮蓝染色、ELISA、Von kossa染色等方法检测其对PDLFs总蛋白量、碱性磷酸酶(ALP)、骨形成蛋白2（BMP-2）及矿化结节形成的影响。 结果 CsA单独及与LPS联合应用可促进细胞总蛋白的合成，增加细胞ALP活性及BMP-2的表达，并促进细胞矿化结节的早期形成；LPS单独应用可增加细胞的ALP活性，但对细胞的总蛋白合成、BMP-2的表达及矿化结节的早期形成无明显影响。。结论 一定浓度的CsA促PDLFs合成、分化作用明显，CsA联合LPS在促进细胞分化方面具有一定的协同作用。

Effects of Cyclosporine A and Porphyromonas gingivalis-lipopolysaccharide on Biological Activity of in vitro Cultured Human Periodontal Ligament Fibroblasts

Objective To evaluate the in vitro effects of cyclosporine A(CsA) and Porphyromonas gingivalis-lipopolysaccharide(Pg-LPS) on the biological activity of human periodontal ligament fibroblasts(hPDLFs).Methods hPDLFs were incubated with CsA 100 ng/mL,Pg-LPS 100 ng/mL,CsA 100 ng/mL+Pg-LPS 100 ng/mL.Then the total protein was extracted and the concentration was assessed with Coomassie brilliant blue.Several features such as expression of bone morphogenetic protein-2(BMP-2) and alkaline phosphatase(ALP) were detected by enzyme-linked immunosorbent assay(ELISA).The formation of mineralized islets was assessed by Von kossa staining.Results CsA alone or plus LPS could significantly enhanced the total protein concentration,ALP,BMP-2 and mineralized islets of hPDLFs;LPS alone could significantly promote cells ALP expression,whereas has no significant effect on protein concentration,BMP-2 and mineralized islets of hPDLFs.Conclusion At certain concentrations,CsA could significantly enhance hPDLFs biological activity,synergistic effect was observed on enhancing cell differentiation when combined with LPS.