| 胰岛素受体底物1在前成骨细胞分化过程中的表达 |
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| 引用本文： | 何玉玲,周后德,隋国良,刘敏,黄秋霞,廖二元.胰岛素受体底物1在前成骨细胞分化过程中的表达[J].中国医科大学学报,2012,41(9):769-772. |
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| 作者姓名： | 何玉玲 周后德 隋国良 刘敏 黄秋霞 廖二元 |
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| 作者单位： | 1. 广西医科大学第一附属医院代谢糖尿病中心,南宁,530021
2. 中南大学湘雅二医院代谢内分泌研究所,长沙,410011 |
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| 基金项目： | 国家自然科学基金资助项目(30400218) |
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| 摘 要： | 目的 研究胰岛素受体底物1(IRS-1)在小鼠前成骨样MC3T3-E1细胞分化过程中的表达.方法 在小鼠前成骨样MC3T3-E1细胞分化成熟过程中,分别在不同时点收集细胞及上清液.骨钙素含量用放射免疫法测定;碱性磷酸酶活性用α-磷酸奈酚法测定;Ⅰ型胶原、骨涎蛋白、骨桥素和IRS-1的mRNA表达应用半定量RT-PCR检测;免疫印迹法测定IRS-1蛋白水平.结果 在小鼠前成骨样MC3T3-E1细胞分化过程中,IRS-1的表达量逐渐增加,而且与Ⅰ型胶原、骨钙素、骨涎素和骨桥素呈正相关.结论 IRS-1参与MC3T3-E1细胞的增殖、成熟与矿化的全过程.
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| 关 键 词： | MC3T3-E1细胞 胰岛素受体底物1 分化 |
The Change of IRS-1 Expression during the Differentiation of MC3T3-E1 Cells |
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| HE Yu-ling
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ZHOU Hou-de
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SUI Guo-liang
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LIU Min
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HUANG Qiu-xia
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LIAO Er-yuan.The Change of IRS-1 Expression during the Differentiation of MC3T3-E1 Cells[J].Journal of China Medical University,2012,41(9):769-772. |
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| Authors: | HE Yu-ling ZHOU Hou-de SUI Guo-liang LIU Min HUANG Qiu-xia LIAO Er-yuan |
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| Institution: | 1.The Diabetes Mellitus and Metabolism Research Center,The First Affiliate Hospital of Guangxi Medical University,Nanning 530021,China;2.Institute of Endocrinology and Metabolism,The Second Xiangya Hospital,Central South University,Changsha 410011,China) |
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| Abstract: | Objective To investigate the expression profile of insulin receptor substrate 1(IRS-1) during the differentiation of preosteoblastic cells.Methods Pnitrophenyl phosphate assay was used to evaluate alkaline phosphatase activity,RIA was used to evaluate osteocalcin.Semi-quantitive RT-PCR was used to examine the mRNA levels of bone sialoprotein(BSP),osteopontin(OPN),collagen type Ⅰ(Col Ⅰ)and IRS-1 in MC3T3-E1 cells.Western blot was adopted to evaluate the level of IRS-1 protein.Results During differentiation of MC3T3-E1 cells,IRS-1 mRNA and protein levels gradually increased.Osteocalcin,Col Ⅰ,OPN and BSP were positively related to IRS-1.Conclusion IRS-1 may be involved in the proliferation,differentiation and mineralization of murine preosteoblastic MC3T3-E1 cells. |
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| Keywords: | murine preosteoblastic MC3T3-E1 cells insulin receptor substrate 1 differentiation |
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