多西他赛对人子宫内膜腺癌Ishikawa细胞凋亡诱导的实验研究

  目的 探讨多西他赛对体外培养的人子宫内膜腺癌Ishikawa细胞凋亡的诱导作用。方法 体外培养Ishikawa细胞，不同浓度的多西他赛作用48 h后，用MTT法和流式细胞术检测多西他赛对Ishikawa细胞增殖和细胞周期的影响。结果 多西他赛对Ishikawa细胞的增殖具有明显抑制作用，呈剂量依赖性（P＜0.05）。多西他赛引起细胞阻滞在G1~S期，细胞凋亡率增加，呈剂量依赖性（P＜0.05）。结论 人子宫内膜腺癌Ishikawa细胞对多西他赛具有药物敏感性，多西他赛对人子宫内膜腺癌Ishikawa细胞的杀伤作用是通过诱导细胞凋亡途径实现的。

Experimental Study on Docetaxel-induced Apoptosis of Human Endometrial Adenocarcinoma Ishikawa Cells

Objective To investigate the in vitro effects of docetaxel on proliferation and apoptosis of cultured human endometrial adenocarcinoma Ishikawa cells.Methods Human endometrial adenocarcinoma Ishikawa cells were cultured in vitro and treated with different concentration of docetaxel for 48 h respectively;MTT assay and flow cytometry were used to measure the effects of docetaxel on cell proliferation and cell cycle.Results Docetaxel inhibited cell proliferation of cultured Ishikawa cells in a dose dependent manner(P < 0.05).Docetaxel blocked the cell cycle in G1-S period and increased the rates of cell apoptosis in a dose dependent manner(P < 0.05).Conclusion Docetaxel is effective for inhibiting the growth of endometrial adenocarcinoma Ishikawa cells.This anti-neoplastic effect may function through cell cycle arrest in G1-S period and increased cell apoptosis.