血小板异常直方图在判断血小板计数结果中的应用价值

目的 探讨血小板异常直方图在判断血小板计数结果中的临床应用价值。方法 利用Sysmex K-4500血细胞分析仪对276例血液标本分析测定，并根据检测结果中血小板直方图、血小板平均体积（MPV）、血小板板分布宽度（PDW）、红细胞平均体积（MCV）和红细胞体积分布宽度（RDW）不同，分为正常血小板组、大血小板组、血小板聚集组、小红细胞干扰组，并对各组血小板进行显微镜手工法计数。结果 正常血小板组两种方法计数结果差异无统计学意义（P〉O．05）；大血小板组与血小板聚集组两种方法计数结果差异有统计学意义（P〈O．01），仪器法结果偏低；小红细胞干扰组两种方法计数结果差异有统计学意义（P〈O．01），仪器法偏高。结论 对于血小板直方图异常的标本应分析原因，重留标本复查或手工法计数进行纠正，为临床提供可靠检验数据。

Clinical application value of abnormal platelet bargraph in the results of platelet count

Objective To investigate the clinical application value of abnormal platelet bargraph in the results of platelet count. Methods 276 cases of blood samples were assayed with Sysmex K-4500 cytoanalyze and divided into orthoplastocyte group, big platelet group, platelet aggregation group, and microcyte interference group according to the difference of platelet bargraph, mean platelet volume （MPV）, platelet distribution width （PDW）, mean corpuscular volume （MCV） and red cell distribution width （RDW）. Platelet count was performed with microscope for all groups. Results There was no significant difference of platelet count between the two methods in orthoplastocyte group （P〉0.05）. The difference of platelet count was significant in big platelet group, platelet aggregation and microcyte interference group between the two methods （P〈0.01）, while the results assayed with instrumental method were recta-low in the former two groups, meta-high in microcyte interference group. Conclusion Reason for platelet bargraph abnormality should be identified, and the sample should be collected and checked once again or perform manual platelet count to correct the mistaken result, so as to offer reliable laboratory data to the clinic