Mechanism of peripheral blood mononuclear cell invasion by HBV on artificial immunization in newborns |
| |
Authors: | Yue Yafei Meng Jinlai Zhang Shulin |
| |
Affiliation: | Department of Obstetrics & Gynecology, The First Hospital of Xi'an Jiaotong University, China. zhangsl451206@163.com |
| |
Abstract: | Objective To study the effect and mechanism of the peripheral blood mononuclear cell (PBMC) invasion by HBV on artificial immunization in newborns Methods Fifty two newborns of HBsAg positive mothers were immunized with HBIG (hepatitis B immunoglobulin) and HBVac (hepatitis B vaccine) and were followed up for 7 months The newborns' HBV DNA in serum and in the PBMCs was detected with nested PCR; anti HBs was tested with solid phase radioimmunoassay (SP RIA) PBMCs isolated from newborn peripheral blood were incubated in the presence of PHA or purified HBsAg Interleukin 2 (IL 2) level in culture supernatants of activated cells was detected by ELISA Results The failure rate of immunization was higher in infants with positive HBV DNA in PBMCs than those with negative HBV DNA ( P <0 05); IL 2 level in PBMC culture supernatants was lower in former than in the latter and in normal controls ( P <0 05) The level of IL 2 in the immunization failure newborns was lower than that in the successfully immunized newborns and in normal controls ( P <0 05) Conclusions Intrauterine invasion of PBMCs by HBV is one of the important reasons for immunization failure in newborns IL 2 production is closely related to the invasion of PBMCs by HBV, which may contribute to the failure of artificial immunization in newborns |
| |
Keywords: | hepatitis B virus transmission peripheral blood mononuclear cell artificial immunization interleukin- 2 |
本文献已被 CNKI 万方数据 PubMed 等数据库收录! |
| 点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息 |
|
点击此处可从《中华医学杂志(英文版)》下载全文 |