Chromosome 14q may harbor multiple tumor suppressor genes in primary glioblastom a multiformec

Objective To evaluate whether deletion of chromosome 14q is involved in the carcinogenesis of primary glioblastoma multiforme and to identify possibly common deletion reg ions. Methods Fourteen fluorescent dye- labeled polymorphic markers were used and polymerase c hain reaction- based microsatellite analysis was employed to investigate loss of heterozygosity (LOH) on chromosome 14q in 20 primary glioblastoma multiforme (G BM). Results Ten of twenty (50%) GBM displayed LOH at one or more of the markers on chromoso me 14q. Five tumors showed either LOH or non- informative on all markers teste d. The most frequent LOH was observed at locus D14S65 (57. 1%) on 14q32. 1, and in the chromosomal region spanning from D14S63 (47. 1%) to D14S74 (46. 7%) on 1 4q23- 31. None of the informative loci exhibited microsatellite instability.Conclusions Allelic deletion on chromosome 14q plays an important role in the pathogenesis o f GBM. Chromosomal regions at locus D14S65 on 14q32. 1 and spanning from D14S63 to D14S74 on 14q23- 31 may harbor multiple tumor suppressor genes associated wi th GBM.