病理性瘢痕中c-fos原癌基因的表达

目的　病理性瘢痕是创伤过度愈合的结果，以成纤维细胞的异常增殖、合成及分泌大量胶原和细胞外基质为特征，其形成机理仍不清楚。探讨原癌基因c-fos的表达与病理性瘢痕形成的相关性。方法　应用免疫组化SP法，检测c-fos蛋白在增生性瘢痕、瘢痕疙瘩及正常皮肤组织中的表达和分布，并用图像定量分析比较其差异。结果　在增生性瘢痕和瘢痕疙瘩的成纤维细胞中c-fos呈强阳性表达，两组间无明显差异，而与正常皮肤对照组均有显著性差异。结论　增生性瘢痕与瘢痕疙瘩中c-fos蛋白表达升高，存在c-fos癌基因的激活，可能参与了成纤维细胞的分化增殖、胶原合成与降解以及对细胞因子的调控，并导致瘢痕增生。

Experimental study of the expression of c-fos proto-oncogene on hypertrophic and keloid scars

Objective　Abnormal scars, including hypertrophic and keloid scars, are the result of over wound-healing, characterized by aberrant proliferation of fibroblasts and the accumulation of excessive collagen and extracellular matrix. The pathogenic mechanisms underlying the two lesions are unclear. The aim is to investigate the correlation between the expression of c-fos proto-oncogene and role in tumorigenic process and abnormal scarring. Methods　Immunohistochemical technique was performed to detect the expression and distribution of c-fos proteins in 50 samples biopsied from 10 cases of hypertrophic scars, 10 cases of keloids and 5 cases of normal skin with antibodies against c-fos. Image analysis was applied to compare their quantitative difference of expression. Results　c-fos expression on hypertrophic and keloid scars was significantly higher than normal skin controls, and there was no difference between the two lesions. Positive expression distributed mainly in the nucleus of fibroblasts of the two lesions. Conclusion　c-fos oncogene are activated on hypertrophic and keloid scars. It may contribute to proliferation and differentiation of fibroblasts, synthesis and degradation of collagen and regulation of cytokines and induce abnormal scarring. The mechanisms of its effects remain a mystery.