大鼠局灶性脑缺血再灌注后Fas表达与细胞凋亡关系的研究

目的 :本实验通过观察缺血再灌注后大鼠脑组织中 Fas的表达及细胞凋亡的情况 ;初步探讨脑缺血后 Fas表达与细胞凋亡的关系 ,以及其发生的机理。方法 :采用 TUNEL和免疫组化方法观察细胞凋亡及 Fas蛋白在脑缺血再灌注后随时间延长动态变化情况 ,并利用图像分析测定二者的免疫强度。结果 :脑缺血再灌注后神经细胞过度地表达 Fas,以缺血半暗带明显 ,且在缺血再灌注后 6小时达高峰 ,于 2 4小时开始下降。而细胞的凋亡于缺血再灌注后 6小时开始增加 ,于 2 4小时达高峰 ,于 36小时略有下降 ,凋亡细胞分布也以缺血半暗带明显。结论 :脑缺血再灌注可引起Fas的过度表达和神经细胞凋亡增加 ,而缺血后 Fas的过度表达是诱导细胞凋亡的重要的因素之一

The relationship between the expression of Fas protein and apoptosis after ischemia and reperfusion

Objective: To study the relationship between the expression of Fas protein and apoptosis after ischemia and reperfusion.Methods: With the immunohistochemisty method to determine Fas expression, and apoptotic cell death was determined by using terminal deoxynucleotidyl trasferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) technique, and picture analyze was used to observe the change of apoptotic cells and Fas protein with the lengthening of reperfusion time. Results: apoptotic cells and Fas protein expressed after ischemia and reperfusion. The expression of Fas peaked at 6h of reperfusion and decreased obviously at 24h after reperfusion. The apoptotic cells peaked at 24h after reperfusion and decreased at 36h. Conclusion: The results mentioned above suggest that injury of cerbral ischemia and reperfusion can induce neuron apoptosis, and the expression of Fas protein can induce the apoptosis after ischemia and may be related to the activation signal of delayed neuron death.