Morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus,type 9 |
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Authors: | U. Jäger H. Gruler B. Bültmann |
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Affiliation: | (1) Abteilung für Allgemeine Physiologie Universität Ulm, Germany;(2) Abteilung für Biophysik Universität Ulm, Germany;(3) Abteilung für Pathologie Universität Ulm, Germany |
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Abstract: | Summary The membrane potential,Em, of human granulocytes (PMNs), was recorded using glass microelectrodes. The membrane potentialEm exhibited potential fluctuations accompanied by characteristic changes of cell shape. The periodic potential fluctuations (7-s, 70-s, and 260-s periodicities) ascertained by the autocorrelation technique, suggested the existence of an internal clock. The chemoattractant f-Met-Leu-Phe (FMLP) had no influence on the periodicities, whereas the amplitudes of the fluctuations were increased by it. Treatment of PMNs with Echo 9 virus also resulted in hyperpolarization. The 70-s periodicity disappeared under virus treatment indicating a virus-induced change of the internal program and loss of chemotactic activity.Abbreviations ACF Autocorrelation function - Echo 9 AB virus Echo virus, type 9, strain A Barty - Em Membrane potential - FMLP N-formyl-methionyl-leucyl-phenylalanine - PMN Human polymorphonuclear granulocyte - t1,t2,t4 Characteristic periodic length - t3 Aperiodic decay time |
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Keywords: | Human granulocytes Shape changes Chemotaxis Membrane potential fluctuations FMLP Echo 9 AB virus |
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