Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province,North of Iran |
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| Authors: | Shirzad GHOLAMI Farhang BABAMAHMOODI Rohallah ABEDIAN Mehdi SHARIF Abbas SHAHBAZI Abdolsattar PAGHEH Mahdi FAKHAR |
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| Affiliation: | 1.Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran;2.Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran;3.Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran;4.Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran |
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| Abstract: | Background:Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern areas. The aim of present study was to identify Trichostrongylus spp. among human population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.Methods:Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.Results:A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.Conclusion:T. colubriformis was the probable most common zoonotic species causing human trichostrongylosis infection in the area |
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| Keywords: | Trichostrongylus colubriformis Trichostrongylus axei ITS2-PCR Human Iran |
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