伤寒杆菌抗原胶体金层析法的研制及应用

目的:建立一种早期、简便、快速、准确的诊断伤寒的检验方法。方法:采用单克隆抗体,胶体金标记技术,运用双抗体夹心法,免疫层析原理,建立伤寒杆菌抗原胶体金层析法。与肥达反应、血培养、ELISA法进行对比。结果:伤寒杆菌抗原试纸条的灵敏度较肥达反应、血培养高,特异性与ELISA法接近,灵敏度比ELISA法稍低。结论:伤寒杆菌抗原试纸条具有简便、快速、结果易懂、无需特殊仪器的优点,能满足急诊检验的需要,提供了便利条件,适合于广大基层门诊推广、体检普查以及流行病学调查。

Study on assay of rapid gold immunochromatography for salmonella typhi antigen detection

Objective:To establish a early,simple,rapid and accurate detecting method for patients with typhoid.Methods:To establish Salmonella typhi bacilli antigen-colloidal gold detection techinique based on double antibody sandwich and immunochromatography by using monoclonal and colloidal gold conjugation techniques.Compare the results with Widal reaction,blood culture and ELISA.Results:The test slip shows a higher sensibility than Widal reaction and blood culture.but a little lower sensibility than ELISA.The test slip specificity is close to ELISA.Conclusion:The method is simple,rapid,of no special apparatus and easy to interpret results which satisfies the emergent diagnosis.It is especially suitable for outpatient clinics,physical examination investigation and epidemiological investigation.