米非司酮对子宫内膜癌细胞周期时相调控的研究

 目的 探讨抗孕激素米非司酮对子宫内膜癌细胞周期时相的调控作用. 方法 体外培养子宫内膜癌HHUA细胞,不同浓度米非司酮处理细胞24～96小时,流式细胞术（FCM）测定癌细胞周期分布的变化;免疫组化法观察细胞周期调控蛋白的表达变化. 结果 当抗孕激素米非司酮浓度≥5μmol/L作用癌细胞36小时,G1期细胞比率明显上升,S期细胞比率（SPF）降低（P〈0.05）;细胞周期调控蛋白p21和p16表达明显上调（P〈0.05）. 结论 抗孕激素米非司酮通过调节细胞周期相关蛋白表达使HHUA细胞阻滞于G1期,抑制癌细胞增殖.

Study on the Controlling of Mifepristone on Cell Cycle of Endometrial Carcinoma

Objective 　To study the effect of antiprogestin mifepristone on cell cycle of endomet rial carcinoma. Methods 　Human endometrial carcinoma HHUA cell was cultured in vitro and t reated with mifepristone in different concent ration for 24-96 hours. Influence of mifepristone on the cell cycle dist ribution were detected by using flow cytometry ( FCM) . The expression of protein related cell cycle was observed by immunohistochemical. Results 　Treated HHUA cell in vit ro with mifepristone ≥5μmol/ L for 36 hours , rate of G1phase was increased and S-phase f raction (SPF) was decreased obviously ( P < 0. 05) . The expression of cell cycle related protein p21 and p16 were raised significantly ( P < 0. 05) . Conclusion Antiprogestins mifepristone could effectively block HHUA cells at G₁ phase and inhibit the proliferation by cont rolling the expression of protein related cell cycle.