PCR法和传统方法对体液标本中致病真菌的检测比较

目的评价PCR方法诊断深部真菌感染的敏感性和特异性。方法应用真菌通用引物ITS4和ITS86对83例临床体液标本中的致病真菌进行PCR法快速检定,并与传统方法检验的结果进行比较。结果直接PCR扩增的阳性率与传统方法差异无统计学意义。结论采用ITS通用引物PCR法可准确、特异、快速、敏感地检测深部致病真菌。

The Comparison of PCR and Traditional Method for Detecting Pathogenic Fungi from Humoral Samples

Objective:To evaluate the sensitivity and specificity of fast PCR diagnostic method for the deep fungal infection Methods Application of fungal ITS4 and ITS86 universal primers on 83 cases with humoral clinical samples for PCR test to de- tect pathogenic fungi,the results were compared with that of traditional methods and analyze statistically.Result The positive rate of PCR test with humoral clinical samples and the traditional method was similar.There was no significant statistical differ- ence.Conclusion To detect the humoral clinical samples with ITS fungal universal primers and PCR test might provide an accu- rate,specific,sensitive,and rapid approach to detect the pathogenic fungi.