血管紧张素转换酶基因多态性及血清水平与脑梗死的关系

目的研究血管紧张素转换酶（ACE）基因多态性及血清ACE水平与脑梗死之间的相互关系及可能机制。方法对84例脑梗死患者和74例健康对照者用聚合酶链反应（PCR）技术和琼脂糖凝胶电泳法分别进行ACE基因插入／缺失（I／D）多态性测定。分析比较脑梗死组与健康对照组之间ACE基因多态性的分布差异。结果脑梗死组DD基因型频率（50％）和D等位基因频率（64％）与健康对照组（分别为28％、4600）比较增高，差异有统计学意义（分别为P〈0．01，P〈0．05）。ACE基因多态性与血清ACE水平有关，ACE水平依次为：DD型〉ID型〉Ⅱ型，三者相互之间差异有统计学意义（P〈0．01）。结论ACE基因多态性与血清ACE水平和脑梗死有关，其DD型基因和D等位基因是脑梗死的危险因素。

Associatian of angiotensin converting enzyme gene polymorphism and its serum levels with cerebral infarction

Objective To investigate the association of angiotensin converting enzyme(ACE) gene polymorphism and its serum levels with cerebral infarction, and further to explore its possible mechanism. Methods Eighty-four patients with cerebral infarction(cerebral infarction group) and 74 healthy controls(healthy control group) underwent the determination of ACE gene insertion/deletion polymorphism by polymerase chain reaction(PCR) and agarose gel electrophoresis(AGE). The serum levels of ACE were measured simultaneously. The difference in distribution of ACE gene polymorphism was compared between cerebral infarction group and ealthy control group. Results The frequency of DD genotype in cerebral infarction group was significantly higher than that of healthy control group(50% vs 28% ,P<0.01); so was the frequency of D allele(64% vs 46% ,P<0.05). ACE gene polymorphism was associated with the serum level of ACE. A significant difference in serum ACE level was observed among three ACE genotypes. The highest serum ACE level or activity was in deletion homozygote(genotype DD), followed by heterzygote(genotype ID) and insertion homozygote(genotype Ⅱ)(P<0.01). Conclusion The polyrnorphism of ACE gene and serum level of ACE are associated with cerebral infarction. The DD gene type of ACE and D allele may be the risk factor for cerebral infarction.