促红细胞生成素对大鼠全脑缺血再灌注后学习记忆障碍的保护作用

目的　探讨促红细胞生成素 (Erythropoietin ,EPO)对全脑缺血再灌注后学习和记忆能力的影响。 方法　阻断大鼠四动脉血流 15min ,再灌注 7天制成全脑缺血再灌注模型 ;于再灌注开始前 ,EPO治疗组经腹腔给予 3 0 0 0U /Kg剂量的EPO ;缺血再灌注组和假手术组给生理盐水注射。再灌注第 7天 ,应用Y型迷宫测定学习和记忆能力 ,然后断头取脑。脑块以石蜡包埋制作HE切片镜检。结果　全脑缺血再灌注后 7天 ,缺血再灌注组可见大量神经细胞坏死 ,EPO治疗组组织学检查仅见少量神经细胞变性坏死 ,脑缺血程度明显轻于缺血再灌注组。与缺血再灌注组相比 ,大鼠学习尝试次数减少 (P <0 .0 5 ) ,记忆测试 10次所用时间缩短 ,正确次数增多 (P <0 .0 5 )。结论　促红细胞生成素能够显著减少细胞死亡、提高大鼠全脑缺血再灌注后学习和记忆能力 ,对脑缺血再灌注有保护作用。

Protective effects of EPO on learning and memory after whole cerebral ischemia-reperfusion in rats

Objective To explore the neuroprotective effects of EPO on learning and memory after whole cerebral ischemia-reperfusion in rats. Methods The model of whole cerebral ischemia-reperfusion was made by occluding four cerebral arteries for 15minutes and reperfusion for 7days. The animals received either EPO (3,000 units/kg of bodyweight) or saline i.p. just before the beginning of reperfusion. In the seventh day,the rats were observed on learning and memory by using Y maze,then the brains were removed and used for paraffin sections,which were used for the observation of cell death with the method of H-E staining. Result In the day after ischemia-reperfusion,there are many neueons necrosis in ischemia-reperfusion group and only a few neueons necrosis in EPO cured group, the extent of ischemia-reperfusion injury in EPO cured group was lower than that in ischemia-reperfusion group. Compared with ischemia-reperfusion group, the number of learning reduced (P<0.05); in memory test the time used for 10 maze tests was markly shorter and the correct times increased(P<0.05). Conclusions EPO could reduce the neurons necrosis and the cerebral infraction volume and improve the ability of learning and memory after whole cerebral ischemia-reperfusion in rats,so EPO has a neuoroprotective effect on ischemia-reperfusion injury.