一种糖尿病-高血压大鼠模型的建立

本实验旨在建立一种糖尿病-高血压大鼠模型。采用Wistar大鼠腹腔注射STZ（链脲佐菌素）诱导糖尿病之后，给予1% NaCl饮水，以无创尾动脉血压测定法进行血压监测。至血压稳定高于正常大鼠50 mmHg后，颈动脉插管法测定血压，测定血浆ET-1、AngⅡ含量；并采用半定量PCR方法对肾脏、肝脏、主动脉血管紧张素原基因表达及前内皮素原基因表达情况进行检测。结果显示，STZ诱导的糖尿病大鼠给予1% NaCl饮水3周，血压开始升高，第6周升至约150 mmHg，并维持5周左右，至第12周血压继续上升到160 mmHg以上。此时，大鼠伴有血浆ET-1含量和肾脏 Ang Ⅱ含量上升，主动脉ET-1和肾脏Ang Ⅱ基因表达上调。因此，STZ诱导糖尿病大鼠饮用1% NaCl可制备一种糖尿病相关的高血压动物模型。

Establishment of a diabetic-hypertensive rat model

This study is to establish a diabetic-hypertensive model in rats. After the induction of diabetes by streptozocin (STZ), rats were maintained with free access to rat chow and 1% NaCl drinking water.  Blood  pressure was monitored at conscious state by tail-cuff weekly till it was 50 mmHg higher than normal animal steadily.  Finally, blood pressure was measured by catheterization of the right carotid artery and plasma ET-1 and Ang II, kidney Ang II and angiotensinogen or preproendothelin gene expression in liver or aorta were assayed separately.  STZ-diabetic rats that maintained with 1% NaCl drinking water exhibited obviously increasing blood pressure since the third week.  Then the pressure reached 150 mmHg at the 6th week and was maintained until the 11th week.  Till the 12th week, the blood pressure reached to higher than 160 mmHg.  In addition, these high blood pressure rats were accompanied with increased blood plasma ET-1 and Ang II and augmented gene expression levels of angiotensinogen in kidneys and preproendothelin in aorta tissues.  Loading sodium chloride chronically to STZ-diabetic rats could prepare a diabetic-hypertensive rat model.