涤纶复合毡型人工韧带的实验研究

目的观察涤纶复合毡型人工韧带在动物体内的生物相容性和生物力学特性。方法用涤纶复合毡型人工韧带替代羊的胫后肌腱,分别于术后3、6、9、12个月收集标本做力学测定,并做光学显微镜观察;将涤纶复合毡型人工韧带植入鼠的四肢肌肉组织内,于术后2、4、8、12周处死,取出的标本做光学显微镜和透射电镜观察。结果替代羊胫后肌腱的涤纶复合毡型人工韧带,早期被纤维组织呈“岛屿”状分隔。随着植入时间延长,韧带周围出现胶原带,与伸入毡型韧带中间的胶原化纤维组织连接成片状,其抗拉强度不随时间的延长而发生衰减,经统计学分析,不同样本间的力学强度差异无显著意义。植入鼠肌肉组织内的毡型韧带早期见肉芽组织由四周向韧带中央呈包绕性生长,“盲区”较大。随着时间的延长,“盲区”逐渐缩小直至消失,被肉芽组织替代,胶原纤维明显增多,形成不同程度胶原化的纤维组织。结论涤纶复合毡型人工韧带富于网状孔隙结构,有利于组织的生长和愈合,具有良好的生物相容性和力学性能,能用于修复和重建人体受损的韧带组织。

Experimental Study of Terylene Polyester Felt Type Artificial Ligament

Objective To study the compatibility in animal and biomechanics characteristic of the terylene polyester felt type artificial ligament.Methods Goats' posterior tibial tendon was substituted by terylene polyester felt type artificial ligament. Specimens were collected 3, 6, 9,and 12 months after operation to measure their mechanical characteristics and to be observed by optical microscope. The artificial ligament was cut into pieces and implanted into rats' muscular tissue of extremities. The rats were executed 2,4,8,and 12 weeks after operation. The specimens were observed by optical and electron microscope.Results After implantation, the terylene polyester felt type artificial ligament was islanded by fibrous tissue at early stage. Then collagen strap was found around ligament and united with collagen component of fibrous tissue which extended into woven fabric type artificial ligament. Its tensile strength showed no attenuation trend over time. There was no significant difference in mechanical strength among different samples. The ligaments implanted into muscular tissue of rats were observed by the optical microscope. At the early stage, the granulation tissue grew from periphery to the center of ligaments. The blind area was relatively large. Then the blind area was gradually lessened till disappearance and was substituted by the granulation tissue. There were less inflammatory cells and macrophages and more collagen fibers which were lined, compacted and formed into fibrous tissue with various degrees collagenization.Conclusion There are abundant holes and network structure in artificial ligament which benefits the growth and the recovery of tissues. It can be used in repair and reconstruction of ligament tissue of human body.