Fragmentation of DNA and chromatin during senescence in barley leaves |
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| Authors: | S G Chen B I Srivastava |
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| Affiliation: | 1. State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing 210096, China;2. Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China;3. The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China;4. Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People''s Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China;5. Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China;6. Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100086, China;7. Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China |
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| Abstract: | DNA or chromatin from young (7 day) first seedling leaves of barley showed only one component which migrated little into 1% agarose gels on electrophoresis. However, DNA or chromatin from senescent (17-, 19-, and 23-day-old) leaves showed additional dispersed components migrating throughout the length of the gel which increased with age. These low molecular weight components increased even more on autodigestion of chromatin from senescent leaves by its associated DNase or by digestion of DNA from senescent leaves with partially purified chromatin DNase. DNA or chromatin from young leaves also produced gel pattern similar to old leaves on digestion with partially purified chromatin DNase from old barley leaves. Thus, fragmentation of DNA and chromatin by chromatin associated DNase, previously shown to increase 4000% on aging, occurs during senescence in barley leaves. |
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