液基细胞学ASC诊断348例的临床分析

目的探讨非典型鳞状上皮细胞（ASC）的临床意义及其进一步处理方法。方法对348例宫颈细胞学检查诊断为ASC的患者行阴道镜下多点活检术，同时采用荧光定量PCR法检测HPVDNA含量。结果组织活检CINⅠ～Ⅲ85例，炎症263例；HPVDNA阳性84例、阴性264例。高危型HPVDNA阳性标本中CINⅡ～Ⅲ的检出率明显高于HPV阴性标本（P〈0．01），高危型HPVDNA的阴性预测值为99．0％。结论ASC患者应联合检测HPV，高危型HPVDNA阳性者应直接行阴道镜活检，进一步明确诊断。高危型HPVDNA检测对宫颈CIN有更好的预测价值。

Clinical analysis on 348 cases of atypical squamous cells diagnosed with liquid based cytology

Objective To explore the clinical significance of atypical squamous cells (ASC) and the further dealing method. Methods Multipoint biopsies under colposcope was applied to the 348 patients. At the same time,the content of HPV-DNA detected with FQ-PCR. Results Totally 85 cases among them were CIN Ⅰ-Ⅲ, 263 cases were determined as imflammation, 84 cases were determined as HPV-DNA-positive, and 264 cases were HPV-DNA negative. Compared with that of the specimens with HPV negative, CIN Ⅰ-Ⅲ detection rate of the specimens of HPV-DNA with high risk was significantly higher (P<0. 01). The negativen predictive value of high-risk type HPV-DNA was 99. 0%. Conclusion The patients with HPV-DNA positive should receive biopsies under colposcope so as to acquire final diagnosis. It is necessary for patients with ASC to receive HPV-DNA detection. Detection of HPV-DNA with high risk may have more forecasting value to cervical intra-epithelial neoplasia (CIN).