RIP1蛋白在卵巢癌中的表达及其对卵巢癌细胞增殖和化疗敏感性的影响

目的:探讨RIP1对卵巢癌细胞增殖和化疗敏感性的影响。方法:通过实时定量PCR法检测RIP1在卵巢癌组织和癌旁组织中的表达；免疫组化方法分析卵巢癌组织和癌旁组织中RIP1蛋白的表达；实时定量PCR法以及Western blot法检测卵巢上皮细胞及卵巢癌细胞中RIP1的表达；通过在SKOV3卵巢癌细胞中敲除RIP1蛋白及在A2780卵巢癌细胞中过表达RIP1蛋白，采用MTT法检测其增殖能力的改变，并联合克隆形成法考察细胞对顺铂敏感性的变化。结果:相比于癌旁组织以及卵巢上皮细胞，RIP1在人卵巢癌组织和卵巢癌细胞中表达有不同程度升高，其中在SKOV3细胞中升高最为显著（P＜0.01），在A2780细胞中不显著（P＞0.05）；在SKOV3细胞中敲除RIP1，96 h后细胞增殖能力明显降低（P＜0.01）；同时，在A2780细胞株中过表达RIP1，72 h后细胞增殖能力明显升高（P＜0.05）。RIP1敲除后，顺铂对SKOV3细胞的杀伤作用明显升高（P＜0.05），同时顺铂的IC50降低；RIP1过表达后，顺铂对A2780细胞的杀伤作用明显降低（P＜0.05），同时顺铂的IC50升高。结论:RIP1作为一种癌基因，能够促进肿瘤细胞的增殖，并降低肿瘤细胞对化疗药物的敏感性。

Expression of RIP1 protein in ovarian cancer and its effect on proliferation and chemotherapeutic sensitivity of ovarian cancer cells

Objective:To investigate of RIP1 on cell proliferation and chemotherapeutic sensitivity in ovarian cancer.Methods:The level of RIP1 in ovarian tumors and ovarian peritumors were evaluated by RT-qPCR.Immunohistochemical assay was applied to analyze the protein expression of RIP1 in ovarian tumors and ovarian peritumors.Furthermore,RT-qPCR and Western blot assay were used to determine the expression of RIP1 in ovarian epithelial cells and ovarian cancer cells.After RIP1 knockdown in SKOV3 cells and RIP1 overexpression in A2780 cells,MTT combined with colony survival assay were applied to measure the proliferation ability and cisplatin sensitivity of the cells.Results:Compared with peritumor and ovarian epithelial cells,the level of RIP1 in ovarian tumor and ovarian cancer cells were increased,especially in SKOV3 cells (P＜0.01),but not significant in A2780 cells (P＞0.05).Under the knockdown of RIP1 in SKOV3 cells,the proliferation of the cells was significantly decreased after 96 h of gene silence (P＜0.01).Meanwhile,overexpression of RIP1 in A2780 cells contributed to increased cell proliferation after 72 h (P＜0.05).The cytotoxicity of cisplatin in RIP1 knockdown SKOV3 cells was significantly increased and with lower IC50 of cisplatin (P＜0.05).However,the cytotoxicity of cisplatin in RIP1 overexpression A2780 cells was significantly decreased and with higher IC50 of cisplatin (P＜0.05).Conclusion:As an oncogene,RIP1 could promote the proliferation of ovarian cells and decrease the chemotherapeutic sensitivity of cancer cells to cisplatin.