| 过表达长链非编码RNA H19对肝癌细胞增殖和自噬的影响及可能机制 |
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| 引用本文： | 韩树坤1,彭雪强2,韩冰3. 过表达长链非编码RNA H19对肝癌细胞增殖和自噬的影响及可能机制[J]. 现代肿瘤医学, 2020, 0(16): 2754-2758. DOI: 10.3969/j.issn.1672-4992.2020.16.005 |
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| 作者姓名： | 韩树坤1 彭雪强2 韩冰3 |
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| 作者单位： | 1.中国医科大学辽阳市中心医院普外科;3.呼吸科,辽宁 辽阳 111000;2.中国医科大学附属第四医院普外科,辽宁 沈阳 110032 |
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| 摘 要： | 目的:研究长链非编码RNA H19(long noncoding RNA H19,H19)对肝癌细胞自噬的影响及可能机制。方法:Real-time PCR检测H19在肝癌组织及相应癌旁组织中的表达;CCK-8分析过表达H19对肝癌细胞增殖的影响;免疫荧光实验和Western blot分析过表达H19对肝癌细胞自噬的影响;Western blot分析过表达H19肝癌细胞的Atg7表达变化以及与Atg7干扰质粒共转染后对肝癌细胞自噬的影响。结果:H19在肝癌组织中表达水平高于癌旁组织;过表达H19后肝癌细胞增殖活力及自噬增强;过表达H19通过上调自噬相关基因Atg7促进肝癌细胞自噬。结论:上调H19促进肝癌细胞增殖与自噬,H19对肝癌细胞自噬的调控可能与Atg7有关。
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| 关 键 词： | 肝癌 H19 Atg7 增殖 自噬 |
Effect and mechanism of long noncoding RNA H19 overexpression on proliferation and autophagy in HCC cells |
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| Han Shukun,Peng Xueqiang,Han Bing. Effect and mechanism of long noncoding RNA H19 overexpression on proliferation and autophagy in HCC cells[J]. Journal of Modern Oncology, 2020, 0(16): 2754-2758. DOI: 10.3969/j.issn.1672-4992.2020.16.005 |
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| Authors: | Han Shukun Peng Xueqiang Han Bing |
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| Affiliation: | 1.Department of General Surgery;3.Department of Respiratory Medicine,Liaoyang Central Hospital,China Medical University,Liaoning Liaoyang 111000,China;2.Department of General Surgery,the Fourth Affiliated Hospital,China Medical University,Liaoning Shenyang 110032,China. |
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| Abstract: | Objective:To explore the regulation of long noncoding RNA H19 (H19) on cell proliferation and autophagy of hepatocellular carcinoma.Methods:Real-time PCR was used to measure the expression of H19 in hepatocellular carcinoma tissues and adjacent tissues.The proliferation level of hepatocellular carcinoma cells was analyzed by CCK-8.Immunofluorescence assay and Western blot were used to analyze autophagy activity.Western blot was used to analyze the expression of Atg7 and the effect of co-transfection with Atg7 on autophagy of hepatocellular carcinoma cells.Results:The expression level of H19 was up-regulated in hepatocellular carcinoma tissues than in the adjacent tissues.Overexpressed H19 could promote cells proliferation and autophagy of hepatocellular carcinoma cells.The expression of Atg7 could be enhanced by overexpressed H19.Conclusion:H19 could promote proliferation and autophagy in HCC cells.The promotion of H19 on autophagy might be related to Atg7. |
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| Keywords: | hepatocellular carcinoma H19 Atg7 proliferation autophagy |
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