| 髓系来源的抑制性细胞在小鼠H22原位种植性肝癌中的表达及其意义 |
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| 引用本文： | 李宝华,' target='_blank'>,孙 晋,张 晨,秦博文,杨 军,孔光耀,李宗芳,' target='_blank'>.髓系来源的抑制性细胞在小鼠H22原位种植性肝癌中的表达及其意义[J].现代肿瘤医学,2020,0(9):1419-1425. |
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| 作者姓名： | 李宝华 ' target='_blank'> 孙 晋 张 晨 秦博文 杨 军 孔光耀 李宗芳 ' target='_blank'> |
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| 作者单位： | 1.西安交通大学第二附属医院科研中心实验室；2.生物诊断与生物治疗国家地方联合工程研究中心；3.病理科；4.普通外科干部病房，陕西 西安 710004 |
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| 基金项目： | National Natural Science Foundation of China(No.81001309）；国家自然科学基金（编号:81001309）；陕西省重点研发计划一般项目-社会发展领域（编号:2018SF-197） |
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| 摘 要： | 目的:比较小鼠H22原位种植性肝癌模型中髓系来源的抑制性细胞（MDSCs）和调节性T细胞（Tregs）的表达，确定主要的免疫抑制性细胞。并探讨主要免疫抑制性细胞在小鼠H22原位种植性肝癌模型中的意义。方法:建立小鼠H22原位种植性肝癌模型，用流式细胞术的方法比较外周血、脾脏、肿瘤组织中MDSCs、Tregs的比例。分析MDSCs与CD4+T细胞、CD8+T细胞、NK细胞、NKT细胞的相关性。通过脾脏切除的方法下调MDSCs的表达，观察对肿瘤存活的影响。结果:MDSCs的比例无论在外周血、脾脏还是肿瘤组织中都显著高于Tregs。MDSCs的比例与CD4+T细胞、CD8+T细胞、NK细胞、NKT细胞呈负相关。下调MDSCs的比例可以显著延长小鼠存活期。结论:小鼠H22原位种植性肝癌中主要的免疫抑制性细胞是MDSCs。MDSCs的高表达预示着不良的小鼠存活期。
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| 关 键 词： | 髓系来源的抑制性细胞 调节性T细胞 H22 肝癌 |
The expression and significance of myeloid-derived suppressor cells in murine H22 orthotopic transplantable hepatoma |
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| Li Baohua,' target='_blank'>,Sun Jin,Zhang Chen,Qin Bowen,Yang Jun,Kong Guangyao,Li Zongfang,' target='_blank'>.The expression and significance of myeloid-derived suppressor cells in murine H22 orthotopic transplantable hepatoma[J].Journal of Modern Oncology,2020,0(9):1419-1425. |
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| Authors: | Li Baohua ' target='_blank'> Sun Jin Zhang Chen Qin Bowen Yang Jun Kong Guangyao Li Zongfang ' target='_blank'> |
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| Institution: | 1.Core Research Laboratory；2.National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy；3.Department of Pathology；4.Department of Surgery，Cadre Ward，the Second Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710004，China. |
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| Abstract: | Objective:To compare the percentages of myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs) and to verify which cell types are the main immune suppressive cells in murine H22 orthotopic transplantable hepatoma.Then，to discuss their significance in hepatoma mice.Methods:Murine H22 orthotopic transplantable hepatoma was established.The percentages of MDSCs and Tregs in peripheral blood，spleen and tumor tissue were compared using flow cytometry.The correlations between MDSCs and CD4+T cells，CD8+T cells，NK cells and NKT cells were analyzed.The effect of down-regulation of MDSCs using splenectomy on tumor survival was also detected.Results:The percentages of MDSCs were much higher than that of Tregs in the peripheral blood，spleen and tumor tissue.The percentages of MDSCs were negatively correlated with that of CD4+T cells，CD8+T cells，NK cells and NKT cells.The down-regulation of MDSCs significantly prolonged tumor survival.Conclusion:The main immune suppressive cell type was MDSCs in murine H22 orthotopic transplantable hepatoma.The higher expression of MDSCs predicted poorer tumor survival. |
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| Keywords: | MDSCs Tregs H22 hepatoma |
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