ANGPT1基因在肺腺癌组织中的表达及其预后价值分析

目的:探索血管生成素1（angiopoietin 1,ANGPT1)在肺腺癌组织中的表达及预后意义。方法:从癌症基因组图谱(TCGA)数据库收集肺腺癌和肺鳞癌数据，并下载ANGPT1基因表达谱资料及临床信息资料。采用t检验明确ANGPT1在肺癌组织和正常组织中的表达差异，χ2检验分析ANGPT1基因表达与肺腺癌患者临床病理特征的相关性，Log-rank检验进行生存分析；利用基因集富集分析（Gene Set Enrichment Analysis,GSEA）方法，预测肺腺癌中可能受ANGPT1调控的相关通路。结果:ANGPT1基因在肺癌组织中低表达；其表达水平与T分期（P=0.013)呈负相关；ANGPT1低表达的肺腺癌患者预后明显差于高表达患者（P=0.006），是影响肺腺癌预后的独立保护因素(P＜0.05)。GSEA结果显示，ANGPT1可能通过调节G2M检查点、DNA损伤修复、凋亡、mTOR信号、MYC信号、有丝分裂纺锤体等细胞增殖相关通路影响肺腺癌的发生发展。结论:ANGPT1是肺腺癌的独立预后保护因素，可能成为肺腺癌的潜在预后标志物和治疗靶点。

Prognostic value analysis of ANGPT1 and the expression of ANGPT1 in lung adenocarcinoma tissues

Objective:To investigate the prognostic significance of angiopoietin 1（ANGPT1) and the exprssion of ANGPT1 in lung adenocarcinoma tissues.Methods:Data of lung adenocarcinoma and lung squamous cell carcinoma with the ANGPT1 gene expression profile data and clinical information were downloaded from the Cancer Genome Atlas（TCGA) database.The difference of ANGPT1 expression in lung cancer tissues and normal tissues was determined by t test.The correlation between ANGPT1 gene expression and clinicopathological features of lung adenocarcinoma patients was analyzed by χ2 test.Log-rank test was used for survival analysis.To predict the pathways that may be regulated by ANGPT1 in lung adenocarcinoma,Gene Set Enrichment Analysis（GSEA) method was used for gene set enrichment analysis.Results:The mRNA level of ANGPT1 in lung cancer tissues was lower than that in normal tissues.Its expression level was negatively correlated with T stage（P=0.013).Patients with low-expression ANGPT1 lung adenocarcinoma had significantly worse prognosis than those with high expression（P=0.006).ANGPT1 was an independent protective factors for adenocarcinoma prognosis（P＜0.05).GSEA results showed that ANGPT1 might affect the development of lung adenocarcinoma by regulating G2M checkpoints,DNA damage repair,apoptosis,and cell proliferation-related pathways such as mTOR signaling,MYC signaling and mitotic spindle.Conclusion:ANGPT1 is an independent prognostic factor for lung adenocarcinoma and might be as a potential prognostic biomarker and therapeutic target for lung adenocarcinoma.