| RNA结合蛋白QKI-5对肾癌细胞增殖和细胞周期的影响 |
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| 引用本文： | 张瑞丽1,' target='_blank'>2,窦嫣然2,艾尼瓦尔·艾木都拉2,熊 健1,包永星2. RNA结合蛋白QKI-5对肾癌细胞增殖和细胞周期的影响[J]. 现代肿瘤医学, 2020, 0(6): 871-876. DOI: 10.3969/j.issn.1672-4992.2020.06.001 |
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| 作者姓名： | 张瑞丽1 ' target='_blank'>2 窦嫣然2 艾尼瓦尔·艾木都拉2 熊 健1 包永星2 |
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| 作者单位： | 1.新疆医科大学第一附属医院昌吉分院博士后工作站，新疆 昌吉 831100;2.新疆医科大学第一附属医院肿瘤中心，新疆 乌鲁木齐 830054 |
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| 基金项目： | National Natural Science Foundation of China（No.81760452）;国家自然科学基金项目(编号:81760452）；国家级课题第62批中国博士后基金面上项目二等资助（编号:2017M623338XB） |
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| 摘 要： | 目的:探讨RNA结合蛋白QKI-5在肾癌中的生物学功能。方法:应用以慢病毒为载体的过表达技术观察过表达QKI-5对肾癌细胞增殖作用的影响及对细胞周期的影响。结果:过表达QKI-5能够抑制肾癌细胞株786-O和A-498细胞的增殖能力、克隆形成能力；流式细胞术结果显示:过表达QKI-5使786-O和A-498细胞产生G1期阻滞，而这种阻滞与周期相关蛋白Cyclin D1表达降低及p21、p27表达增高有关。结论:QKI-5在肾癌中发挥着抑癌基因的功能，可以抑制肾癌细胞增殖，并使细胞周期阻滞在G1期。
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| 关 键 词： | RNA结合蛋白QKI-5 肾癌 细胞周期 |
RNA binding protein QKI-5 effect on the proliferation and cell cycle of renal cancer cell |
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| Zhang Ruili1,' target='_blank'>2,Dou Yanran2,Ainiwaer·Aimudula2,Xiong Jian1,Bao Yongxing2. RNA binding protein QKI-5 effect on the proliferation and cell cycle of renal cancer cell[J]. Journal of Modern Oncology, 2020, 0(6): 871-876. DOI: 10.3969/j.issn.1672-4992.2020.06.001 |
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| Authors: | Zhang Ruili1 ' target='_blank'>2 Dou Yanran2 Ainiwaer·Aimudula2 Xiong Jian1 Bao Yongxing2 |
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| Affiliation: | 1.Postdoctoral Workstation,Changji Branch Hospital of the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Changji 831100,China;2.Cancer Center,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China. |
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| Abstract: | Objective:To analyze the function of QKI-5 in renal cell carcinoma（RCC).Methods:Overexpression technique using lentivirws as a vector detected the effect of overexpressing QKI-5 on proliferation and cell cycle of RCC.Results:In vitro assays revealed that the ectopic expression of QKI-5 effectively inhibited cellular proliferation,resulting in significant inhibition of the cellular growth rate and reduction in the colony forming ability of the cells.Moreover,ectopic expression of QKI-5 in 786-O and A-498 cells significantly increased the proportion of cells in G1 phase and decreased the proportion in S phase and displayed a reduction in Cyclin D1 and an accumulation of p21 and p27.Conclusion:Our data establish a suppressive role for QKI-5 in RCC tumourigenesis. |
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| Keywords: | QKI-5 RCC cell cycle |
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