绵羊肝脏、肺脏细粒棘球蚴原头节差异蛋白质表达分析

目的 探讨绵羊肝脏和肺脏细粒棘球蚴原头节差异蛋白质表达谱,为虫体生长、发育调控特异性蛋白筛选奠定基础。方法 提取寄生于绵羊肝脏与肺脏棘球蚴原头节蛋白质样本进行 SDS-PAGE 分析后,利用iTRAQ和液相色谱法以及串联质谱分析(LC-MS/MS)寄生于绵羊肝脏和肺脏细粒棘球蚴原头节蛋白质表达谱,通过Mascot软件和GO软件分析寄生于绵羊肝脏和肺脏棘球蚴原头节的差异表达蛋白质。结果 本研究共鉴定了1 229个有意义的蛋白质点,通过肝脏与肺脏比较有无差异性(肝脏原头节 VS肺脏原头节)和调节趋势后获得217个差异表达蛋白质,在肝脏上调蛋白74个(脂肪酸绑定蛋白、ɑ纤维蛋白原、环氧化物酶、过氧化氢酶、酰基COA合成酶、载脂蛋白A4、磷酸化酶、6-磷酸葡萄糖内脂酶、乙酰辅酶酰基转移酶、还有一些未鉴定的蛋白等);下调蛋白143个(乳酸脱氢酶、谷胱甘肽过氧化物酶、内质网氧化还原酶、肌动蛋白、肌球蛋白、天冬酰胺酶、铁蛋白、6-磷酸葡萄糖酸脱氢酶、热休克蛋白90、胶原纤维I,胶原纤维IV、波形蛋白、玻连蛋白、磷脂转运蛋白、磷酸甘油酸激酶)。结论 本研究发现寄生于绵羊肝脏和肺脏棘球蚴原头节蛋白质表达存在差异。

Differential protein expression analysis on protoscolex of Echinococcus granulosus in the liver and lung of sheep

To study the differential protein expression profile of the protoscolex of Echinococcus granulosus in liver and lung of sheep. In order to screening specific proteins for development and growth regulation of protoscolex. The protein was extracted from protoscolex in liver and lung of sheep from different groups and analyzed the protein profile by SDS-PAGE. And then the protein expression profiles of protoscolex between liver and lung were analyzed by iTRAQ and 2D liquid chromatography-tandem mass spectrometry (LC-MS). The differentially expressed proteins were analyzed by Mascot and GO software. In this study, a total of 1 229 meaningful protein spots were identified. Differentially expressed proteins total 217 were obtained to compare Hepatic_P with Lung_P and the trend of regulation, of which 74 were up-regulated (eg, Fatty acid binding protein, Fibrinogen alpha, Epodide hydrolase, catalase, avyl-COA sythetase, apolipoprotein A4, glycogen phosphorylase, 6-phosphogluconolactonase, acetyl-coenzyme acylthransferse and some uncharacterized proteins, and so on) and 143 were down-regulated (eg, L-lactate dehydrogenase, glutathione peroxidase, endoplasmic reticulum oxidoreductase, actin protein, myosin protein, asparaginase, ferritin, HSP90, 6-phosphoglucoate dehydrogenase, I collagen, IV collagen, vimentin, vitronectin, phospholipid transfer protein, phosphoglycerate kinase and so on). In conclusion,it was found that the protein expression of the protoscolexof E. granulosus was different in sheep liver and lung.