FXR和CDX2在胃黏膜肠化生及胃癌中的表达及意义

目的:探讨法尼酯衍生物X受体(farnesoid X receptor,FXR)和尾型同源盒2(caudal type homeobox 2，CDX2)在胃黏膜肠化生(intestinal metaplasia，IM)及胃癌中的表达和意义。方法:采用免疫组织化学染色法检测FXR和CDX2在30例慢性胃炎、50例IM、60例胃癌组织中的表达。利用卡方检验比较各组间FXR和CDX2的表达差异，并分析其与肠化生和胃癌患者临床病理参数的关系。利用Spearman秩相关检验分析FXR和CDX2表达的相关性。结果:IM和胃癌组织中FXR的高表达率分别为58.0%和38.3%，较慢性胃炎组织(13.3%)均显著增高（P＜0.05）。与IM组织相比，胃癌组织中FXR表达显著下降（P=0.040）。FXR高表达与IM患者肠化生程度较重（中重度）相关（P=0.025）。FXR高表达与胃癌患者分化较好（中高分化）相关（P=0.003）。IM和胃癌组织中CDX2的高表达率分别为46.0%和26.7%，较慢性胃炎组织(6.7%)均显著增高（P＜0.05）。与IM组织相比，胃癌组织中CDX2表达显著下降（P=0.035）。CDX2高表达与IM患者肠化生程度较重（中重度）相关（P=0.004）。CDX2高表达与胃癌患者分化较好（中高分化）相关（P<0.001）。FXR和CDX2表达在慢性胃炎、IM、胃癌组织中均显著正相关（P<0.001）。结论:FXR和CDX2在IM和胃癌组织中表达均上调且显著正相关，可能共同参与了IM和胃癌的发生发展。

Expression and significance of FXR and CDX2 in gastric mucosal intestinal metaplasia and gastric carcinoma

Objective:To investigate the expression and significance of farnesoid X receptor (FXR) and caudal type homeobox 2(CDX2) in the gastric mucosal intestinal metaplasia (IM) and gastric carcinoma.Methods:Immunohistochemical staining was used to detect the expression of FXR and CDX2 in 30 cases of chronic gastritis,50 cases of IM and 60 cases of gastric cancer.The chi-square test was used to compare the expression differences of FXR and CDX2 in each group,and the relationship between the expression of FXR and CDX2 and the clinicopathological parameters of intestinal metaplasia and gastric cancer patients was analyzed.Spearman rank correlation test was used to analyze the correlation between the FXR and CDX2 expression.Results:The high expression rates of FXR in IM and gastric cancer tissues were 58.0% and 38.3%,respectively,which were significantly higher than those in chronic gastritis (13.3%) (P<0.05).Compared with IM tissues,FXR expression was significantly decreased in gastric cancer tissues (P=0.040).High expression of FXR was associated with IM degree (moderate to severe of degree)(P=0.025) of patients with IM.High expression of FXR was associated with differentiation status (moderate or well of differentiation) (P=0.003) of patients with gastric cancer.The high expression rates of CDX2 in IM and gastric cancer tissues were 46.0% and 26.7%,respectively,which were significantly higher than those in chronic gastritis (6.7%) (P<0.05).Compared with IM tissues,CDX2 expression was significantly decreased in gastric cancer tissues (P=0.035).High expression of CDX2 was associated with IM degree (moderate to severe of degree) (P=0.004) of patients with IM.High expression of CDX2 was associated with differentiation status (moderate or well of differentiation) (P＜0.001) of patients with gastric cancer.There was a significant positive correlation between FXR and CDX2 expression in chronic gastritis,IM and gastric cancer tissues (P<0.001).Conclusion:FXR and CDX2 are up-regulated in IM and gastric cancer tissues and have a significant positive correlation.They may be involved in the occurrence and development of IM and gastric cancer.