PARP1在三阴性乳腺癌组织中的表达及其与EMT的相关性

目的:探讨PARP1在三阴性乳腺癌（TNBC）组织中的表达及其与上皮间质转化（EMT）的相关性。方法:收集60例TNBC及30例非TNBC组织，采用免疫组织化学方法检测PARP1的表达水平，并检测60例TNBC组织中E-cadherin和Vimentin的表达水平，分析PARP1、E-cadherin和Vimentin在TNBC中的表达与患者临床病理参数之间的关系，并分析PARP1与E-cadherin和Vimentin的相关性。结果:PARP1在TNBC中的表达明显高于非TNBC。PARP1的表达与TNBC患者的肿瘤大小、淋巴结转移及临床分期有关（P<0.05），与年龄及组织学分级无关。PARP1的表达与E-cadherin的表达呈负相关(P<0.05)，与Vimentin的表达呈正相关(P<0.05)。结论:三阴性乳腺癌组织中存在PARP1高表达，PARP1可能通过调控 E-cadherin和Vimentin的表达促进TNBC上皮间质转化。

Expression of PARP1 in triple negative breast cancer and its relationship with EMT

Objective:To explore the expression of PARP1 in triple negative breast cancer(TNBC) and its relationship with epithelial-mesenchymal transition(EMT)．Methods:Immunohistochemical assay was performed to detect the expression of PARP1 in 60 cases of TNBC and 30 cases of non-TNBC，and also detect E-cadherin and Vimentin in 60 cases of TNBC.Then the association between PARP1，E-cadherin，Vimentin expressions and clinicopathological factors of TNBC as well as the correlation between among PARP1，E-cadherin，Vimentin expressions were analyzed.Results:The positive expression rate of PARP1 was significantly higher in the TNBC than that in the non-TNBC.The expression of PARP1 in the TNBC was associated with the tumor size，lymphatic metastasis and clinical stage(P<0.05)，but no relationship with age and histological grade was observed．The expression of E-cadherin in the TNBC was negatively co-related to that of PARP1(P<0.05)．In contrast，the expression of Vimentin in the TNBC was positively co-related to that of PARP1(P<0.05)．Conclusion:PARP1 was highly expressed in TNBC.PARP1 may contribute to the onset of EMT in the TNBC by regulating the expression of E-cadherin and Vimentin.