江苏省张家港市2017年人源沙门菌血清型特征及分子分型

目的 分析2017年张家港检出的92株人源沙门菌的血清学特征,用脉冲场凝胶电泳（PFGE）技术对优势血清群进行分子分型研究,为该地区沙门菌菌型库积累数据。方法 收集和提取2017年张家港地区人源沙门菌患者血清学特征,对优势群B群沙门菌进行PFGE分子分型检测,利用PulseNet China网络获得相关性指纹图谱并进行聚类分析。结果 92株沙门菌分离株分布7个血清群和16个血清型,以B群为主（33.70％）,30株B群沙门菌PFGE指纹图谱可分为26个基因型（相似值100% 为同一PFGE 型别）,19个克隆系（相似度在0.85~1.00的为同一PFGE的克隆系）。其中9株德尔卑沙门菌可分为5个克隆系7个基因型,7个基因型相似度60.92％~100％;7株阿贡那沙门菌PFGE指纹图谱5个克隆系6个基因型,6个基因型相似度0~100%;6株鼠伤寒沙门菌可分为3个克隆系5个基因型,5个基因型相似度48.51％~100％。结论 张家港市人源沙门菌血清型多样化分布,B群沙门菌为优势血清群。沙门菌PFGE带型较多,基因多态性较高,同一血清型可获得不同PFGE指纹图谱。其中德尔卑沙门菌亲缘关系近,鼠伤寒部分菌株存在较近的亲缘关系,阿贡那沙门菌各克隆系存在明显差异,亲缘关系较远。

Serotypes and molecular typing analysis of human Salmonella in Zhangjiagang,Jiangsu, 2017

Objective To analyze the serotypes characteristics of human Salmonella in Zhangjiagang in 2017 through molecular typing of dominant serogroups determined by pulsed-field gel electrophoresis (PFGE), and accumulate the data for Salmonella type libraries in the region. Methods In 2017, the serological characteristics of human Salmonella in Zhangjiagang area were collected and extracted, PFGE molecular typing was carried out for the dominant group B Salmonella, and the correlation fingerprint was obtained and clustered by PulseNet China network.Results 92 strains of Salmonella isolates belong to 16 serotypes in 7 serogroups, dominated with serogroup B (33.70%), and 30 strains of serogroup B Salmonella PFGE fingerprints can be divided into 26 genotypes (similar values 100% for the same PFGE type) and 19 clones (clone lines of the same PFGE with similarity between 0.85-1.00). Among them, 9 strains of S.derby can be divided into 5 clones and 7 genotypes with the similarity of 60.92%-100%; 7 strains of PFGE fingerprints of S.agona were 5 clones and 6 genotypes with the similarity of 0-100%; 6 strains of S.typhimurium can be divided into 3 clones and 5 genotypes, with the similarity of 48.51%-100%. Conclusion The serotypes of human Salmonella in Zhangjiagang are diverse, and the Salmonella serogroup Bis the dominant serogroup. The results of PFGE molecular typing showed there were many PFGE bands and high gene polymorphism in Salmonella. Different PFGE fingerprints could be obtained from the same serotype. Among them, the phylogenetic relationship between S.derby was close, and some strains of S.typhimurium were closely related. There were significant differences in the clonal lines of S.agona, and the genetic relationship was far.