3D打印个体化钛网的机械力学性能及生物相容性分析

目的:比较3D打印钛网与成品钛网的机械力学性能差异,并评价3D打印钛网对细胞生长及分化的影响.方法:采用激光打印技术制备个体化3D打印钛网,用静态拉伸压缩载荷实验检测机械力学性能,评估机械力学性能.选择4周龄雄性SD大鼠骨髓间充质干细胞,与不同孔径大小的3D打印钛网共培养.采用CCK-8法检测各组细胞增殖情况;对各组细胞进行成骨诱导分化后,检测碱性磷酸酶(ALP)活性改变,运用实时荧光定量PCR检测相关成骨基因的表达;通过扫描电镜及活/死细胞染色,观察细胞在3D打印钛网上的黏附和生长情况.采用SPSS22.0软件包对数据进行统计学分析.结果:3D打印钛网的拉伸压缩负载实验结果显示其具有优异的力学性能;相比对照组,不同孔径钛网对细胞增殖的影响不大,细胞在钛网上显现出良好的黏附和生长状态.3D打印钛网对成骨分化具有一定的促进作用.结论:3D打印钛网的机械力学性能优异,且具有良好的生物相容性.

Mechanical properties of 3D-printed titanium mesh and its biocompatibility in vitro

PURPOSE: To compare the mechanical properties of 3D-printed titanium meshes and pre-shaped titanium meshes, and to evaluate the effects of 3D-printed titanium meshes on cell proliferation and differentiation. METHODS: 3D- printed titanium meshes were produced and prepared with laser printing machine. The mechanical properties were analyzed by static tension and compression load test. Bone marrow mesenchymal stem cells (BMSCs) were extracted from 4-week-old male SD rats. BMSCs were co-cultured with 3D-printed titanium meshes of different apertures. Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. Alkaline phosphatase (ALP) activity assay was used to test ALP activity. The expression of related osteogenic genes was tested by real-time PCR. The adhesion and growth of BMSCs were investigated by scanning electron microscopy (SEM) and living / dead cell staining. SPSS 22.0 software package was used for statistical analysis of the results. RESULTS: The results of 3D-printing Ti-meshes tension and compression loading experiment were excellent. The 3D-printing Ti-meshes showed no inhibitory effects on cell proliferation, survival and adhesion, but had a positive effect on osteogenesis of BMSCs. CONCLUSIONS: The mechanical properties of 3D-printed Ti-meshes are excellent. The 3D-printed Ti-meshes have good biocompatibility.