肾衰营养胶囊对肾性骨病大鼠骨代谢及股骨组织 BMP-2表达的影响

摘要：目的 观察中药复方肾衰营养胶囊对肾性骨病（ROD）模型大鼠骨代谢及股骨组织骨形态发生蛋白 2 （BMP-2）表达的影响。方法 40只雄性SD大鼠随机分为对照组（10只）和造模组（30只）。造模组包括模型组、骨化 三醇组和肾衰营养胶囊组（各10只），造模组大鼠均进行5/6肾切除并给予高磷饮水诱导肾性骨病模型，骨化三醇组 按0.01 mg/kg灌服骨化三醇悬浊液，肾衰营养胶囊组按1.2 g/kg灌服肾衰营养胶囊悬浊液，模型组和对照组灌服等量 生理盐水，共进行12周。检测各组大鼠体质量及给药前后血肌酐（Scr）、尿素氮（BUN）、血钙（Ca2+）、血磷（P3-）、血清 碱性磷酸酶（ALP）、血清全段甲状旁腺激素（iPTH）含量，采用双能X射线检测大鼠股骨骨密度（BMD），Western blot 检测股骨组织BMP-2的表达水平。结果 与模型组和骨化三醇组相比，肾衰营养胶囊组大鼠体质量明显升高，肾功 能指标血BUN、Scr 水平明显降低，骨代谢指标血Ca2+水平升高，血P3-、ALP、iPTH水平明显降低（P＜0.05）；且在给予 肾衰营养胶囊治疗后，大鼠的股骨BMD及BMP-2的表达均较模型组明显升高（P＜0.05）。结论 肾衰营养胶囊能明 显改善5/6肾切除合并肾性骨病模型大鼠骨代谢，延缓肾性骨病的进展，其机制可能与上调BMP-2的表达有关

The effect of Shenshuai Nutrition Capsule on bone metabolism and expression of BMP-2 in rats with renal osteodystrophy

Abstract：Objective To observe the effect of Chinese herbal compound Shenshuai Nutrition Capsule (SSYYJN) on bone metabolism and BMP-2 expression in bone tissue of rats with renal osteodystrophy (ROD). Methods Forty male SD rats were randomly divided into the control group (n=10) and the modeling group (n=30). Rats of modeling group were treated with 5/6 nephrectomy and high-phosphorus drinking water to induce renal osteodystrophy. Then they were randomly divided into model group, calcitriol (VD) group and SSYYJN group (n=10 for each group). The VD group was administered intragastrically with calcitriol suspension at 0.01 mg / kg, and the SSYYJN group was administered intragastrically with SSYYJN suspension at 1.2 g / kg. Model group and control group were given the same amount of normal saline. The intervention lasted for 12 weeks. Then, the body weight and the serum levels of creatinine (Scr), blood urea nitrogen (BUN), blood calcium (Ca2+), blood phosphorus (P3-), alkaline phosphatase (ALP) and immunoreactive parathyroid hormone(iPTH) concentration were measured before and after treatment in three groups of rats. Femur bone mineral density (BMD) was detected by dual-energy X-ray, and the expression of bone morphogenetic protein-2 (BMP-2) was detected by Western blot (WB) assay. Results Compared with model group and VD group, the body weight was markedly increased in SSYYJN group, the BUN and Scr levels, which indicated renal function, were remarkably reduced. For bone metabolism indexes, the blood Ca2+ levels were increased, and the levels of blood P3- , ALP and iPTH were markedly reduced. All the above differences were statistically significant (P＜0.05). After treatment with SSYYJN, the femur BMD and the expression of BMP-2 were significantly higher than those in the model group (P＜0.05). Conclusion Shenshuai Nutrition Capsule can significantly improve bone metabolism in rats with 5/6 nephrectomy and renal osteodystrophy and delay the progression of renal osteodystrophy, which may be related to the up-regulation of BMP-2 expression