结肠癌中LZTS1和CDK1表达与术后辅助化疗敏感性的关系

目的:探讨结肠癌组织中亮氨酸拉链肿瘤抑制基因1(LZTS1)和细胞周期蛋白依赖性激酶1(CDK1)表达与术后辅助化疗敏感性之间的关系。方法:采用免疫组织化学法检测结肠癌组织和癌旁组织中LZTS1和CDK1表达,分析其表达与患者临床病理特征的关系。统计术后辅助化疗后复发转移或死亡患者及未转移患者人数无病生存期(DFS),分析LZTS1和CDK1表达与术后辅助化疗敏感性的关系。Kaplan-Meier法分析LZTS1和CDK1表达对结肠癌患者术后辅助化疗后DFS影响,Cox比例风险回归模型分析影响结肠癌患者术后辅助治疗敏感性的因素。结果:与癌旁组织相比,结肠癌组织中LZTS1阳性表达率显著降低(P<0.05),CDK1阳性表达率显著提高(P<0.05)。LZTS1和CDK1表达与结肠癌患者年龄、性别以及肿瘤直径大小无关(P>0.05),与肿瘤分化程度和淋巴结转移相关(P<0.05)。LZTS1阳性表达患者对术后辅助化疗敏感性高,而CDK1阴性表达患者对术后辅助化疗敏感性高。LZTS1阳性表达患者DFS较阴性表达患者升高(P<0.05),中位生存时间延长。CDK1阳性表达患者DFS较阴性表达患者降低(P<0.05),中位生存时间缩短。Cox多因素分析结果显示,淋巴结转移、LZTS1和CDK1是结肠癌患者术后辅助化疗敏感性的预测指标。结论:结肠癌组织中LZTS1阳性表达降低,CDK1阳性表达升高,LZTS1阳性表达和CDK1阴性表达患者更能从辅助化疗中获益,均可作为术后化疗敏感性的参考指标。

Relationships between expressions of LZTS1 and CDK1 and sensitivity of postoperative adjuvant chemotherapy in colon cancer

Objective:To investigate the relationships between the expressions of leucine zipper tumor suppressor gene 1 (LZTS1) and cyclin-dependent kinase 1 (CDK1) in colon cancer tissues and the sensitivity of postoperative adjuvant chemotherapy.Methods:The expressions of LZTS1 and CDK1 in colon cancer tissues and adjacent tissues were detected by immunohistochemical method,and the relationships between the expressionswith clinicopathological characteristics were analyzed.The relationship between the expression of LZTS1 and CDK1 and the sensitivity of postoperative adjuvant chemotherapy was analyzed by counting the number of patients with recurrence,metastasis or death after adjuvant chemotherapy and the number of patients with metastases.The effects of LZTS1 and CDK1 expression on DFS after adjuvant chemotherapy in patients with colon cancer were analyzed by Kaplan-Meier.Cox proportional hazards regression model was used to analyze the factors influencing the sensitivity of postoperative adjuvant therapy in colon cancer patients.Results:Compared with the adjacent tissues,the positive expression rate of LZTS1 in colon cancer was significantly lower (P＜0.05),and the positive expression rate of LZTS1 in colon cancer was significantly higher (P＜0.05).The expressions of LZTS1 and CDK1 were not correlated with age,gender and diameter of colon cancer (P＞0.05),but with the degree of differentiation and lymph node metastasis (P＜0.05).Patients with positive LZTS1 expression were more sensitive to postoperative adjuvant chemotherapy,while patients with negative CDK1 expression were more sensitive to postoperative adjuvant chemotherapy.Patients with LZTS1 positive expression had higher DFS than patients with negative expression (P＜0.05),and the median survival time was prolonged.Patients with positive CDK1 expression had lower DFS than patients with negative expression (P＜0.05),and the median survival time was shortened.Cox multivariate analysis showed that lymph node metastasis,LZTS1,and CDK1 were predictors of sensitivity to postoperative adjuvant chemotherapy in patients with colon cancer.Conclusion:The positive expression of LZTS1 was decreased in colon cancer tissues,and the positive expression of CDK1 was increased.Patients with positive expression of LZTS1 and negative expression of CDK1 were more likely to benefit from adjuvant chemotherapy.And both could be used as a reference index for postoperative chemotherapy sensitivity.