LncRNA PVT1抑制miR-497-5p表达调控子宫内膜癌细胞增殖和迁移侵袭的分子机制研究

目的:研究长链非编码RNA PVT1和miR-497-5p在子宫内膜癌组织中的表达以及其对癌细胞增殖、迁移和侵袭的影响，并探讨其机制。方法:运用实时荧光定量法测定子宫内膜癌组织、癌旁正常组织及子宫内膜癌细胞HEC-1-B中PVT1和miR-497-5p的表达。用脂质体法将siRNA-PVT1和miR-497-5p mimic转染至子宫内膜癌细胞HEC-1-B；MTT法、Transwell法检测HEC-1-B细胞的增殖、迁移和侵袭。Targetscan在线分析网站预测和双荧光素酶报告基因实验验证LncRNA PVT1和miR-497-5p的靶向关系。将siRNA-PVT1和miR-497-5p inhibitor共转染至HEC-1-B细胞，MTT法、Transwell法检测HEC-1-B细胞的增殖、迁移和侵袭。结果:与癌旁正常组织相比，子宫内膜癌组织中PVT1表达显著上调，miR-497-5p表达显著下调（P<0.05）。沉默PVT1、过表达miR-497-5p均可抑制HEC-1-B细胞的增殖、迁移和侵袭（P<0.05）。PVT1靶向miR-497-5p。抑制miR-497-5p的表达可逆转沉默PVT1对HEC-1-B细胞增殖、迁移和侵袭的抑制作用。结论:沉默PVT1可抑制子宫内膜癌细胞增殖、迁移和侵袭，其机制可能与PVT1靶向miR-497-5p有关，将可为子宫内膜癌的靶向治疗提供新靶点。

Mechanism of LncRNA PVT1 on proliferation，migration and invasion of endometrial cancer cells by inhibiting miR-497-5p

Objective:To study the expression of long non-coding RNA PVT1 and miR-497-5p in endometrial carcinoma tissues and the effects of PVT1 and miR-497-5p on the proliferation，migration and invasion in endometrial carcinoma cells，and to explore the mechanism.Methods:The expressions of PVT1 and miR-497-5p in endometrial cancer tissues，paracancer normal tissues and endometrial cancer cells HEC-1-B were determined by real-time fluorescence quantitative method.siRNA-PVT1 and miR-497-5p mimic were transfected into HEC-1-B endometrial cancer cells by liposome assay.The proliferation，migration and invasion of HEC-1-B cells were detected by MTT assay and transwell assay.Targetscan online analysis site prediction and dual luciferase reporter gene assay verified the targeting relationship between LncRNA PVT1 and miR-497-5p.After cotransfection with siRNA-PVT1 and miR-497-5p inhibitor into HEC-1-B，changes in the proliferation，migration and invasion ability of cancer cells were observed by MTT assay and Transwell assay.Results:Compared with normal paracancer tissues，PVT1 expression was up-regulated and miR-497-5p expression was down-regulated in endometrial carcinoma tissues.Silencing PVT1 and overexpression of miR-497-5p inhibited the proliferation，migration and invasion of HEC-1-B cells.PVT1 targeted miR-497-5p.Inhibition of miR-497-5p expression reverses the inhibitory effect of silencing PVT1 on the proliferation，migration and invasion of HEC-1-B cells.Conclusion:Silencing PVT1 can inhibit the proliferation，migration and invasion of endometrial cancer cells，and its mechanism is related to PVT1 targeting miR-497-5p，which will provide a new target for the targeted treatment of endometrial cancer.