蛇床子素对胃BGC-823细胞的体外放疗增敏作用的实验研究

目的:研究中药单体蛇床子素（Ost）对胃BGC-823细胞的体外放疗增敏作用。方法:采用MTT法检测单药及联合放疗对BGC-823细胞的作用；细胞克隆形成实验检测Ost对BGC-823细胞的放疗增敏作用；流式细胞术分析Ost及放疗对BGC-823细胞的细胞周期及细胞凋亡的影响。结果:Ost对BGC-823细胞具有增殖抑制作用；低细胞毒剂量的Ost（15 μg/ml）对BGC-823细胞具有放疗增敏作用，经单击多靶模型拟合后，Ost联合放疗组细胞存活曲线较单纯放疗组左移，D0、Dq值变小，放疗增敏比SER=1.64。结论:低细胞毒剂量的Ost（15 μg/ml）对BGC-823细胞具有放疗增敏作用，其机制可能与增加放疗诱导的细胞凋亡，引起放疗敏感时相G1期细胞阻滞，减少放疗相对抗拒时相S期细胞比例相关。

Enhancement of radiation effects by Osthole on human gastric BGC-823 cell line in vitro

Objective:To study the enhancement of radiation effects by Osthole on gastric BGC-823 cells in vitro.Methods:MTT assay detected the effects of single drug and combined radiotherapy on BGC-823 cells.Cell clone formation assay detected the enhancement of radiation effects by Ost on BGC-823 cells.Flow cytometry analyzed the effects of Ost and radiotherapy on cell cycle and apoptosis in BGC-823 cells.Results:Ost could inhibit the proliferation of BGC-823 cells.Low cytotoxic dose of Ost (15 μg/ml) had radiotherapy sensitization effect on BGC-823 cells.After single-click multi-target model fitting，the cell surviving fraction of Ost combined radiotherapy group was shifted to the left，compared with the radiotherapy group，and D0 and Dq values were reduced，and the radiotherapy sensitization ratio SER=1.64.Conclusion:Low cytotoxic dose of Ost (15 μg/ml) has radiotherapy sensitization effect on BGC-823 cells.The mechanism may be related to increasing radiation-induced apoptosis，causing cell block in phase G1 when there is radiotherapy sensitization，and reducing the proportion of cells in phase S when there is radiotherapy resistance.