雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症小鼠模型局部牙槽骨破坏的影响

目的：探讨雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症（PMO）小鼠模型局部牙槽骨破坏的影响。方法：无特定病原级雌性成熟Wistar小鼠,适应性喂养7 d 后,随机分为空白组（保留两侧卵巢,只切除卵巢组织周围与卵巢重量相当的脂肪组织）、对照组（切除两侧卵巢+口腔正畸结扎丝结扎）、实验组（切除两侧卵巢+口腔正畸结扎丝结扎+雷洛昔芬试剂）,每组各15只。术后4周拍摄CT,检测骨密度和骨吸收情况,体视显微镜观察牙槽骨破坏情况,酶联免疫吸附测定法（ELISA）检测破骨相关因子白细胞介素1(IL-1)、IL-1α、转化生长因子(TGF)、肿瘤坏死因子α(TNF-α)、TNF-β的表达水平。采用SPSS 18.0软件包对数据进行统计学分析。结果：对照组新骨形成少于实验组。亚甲蓝染色结果显示,对照组小鼠骨显著吸收,体视显微镜下可见骨破坏,实验组小鼠骨吸收程度较轻。与空白组相比,对照组、实验组牙槽骨密度降低而牙槽骨丧失、IL-1、IL-1α、TGF、TNF-α、TNF-β显著升高（P<0.05）;对照组牙槽骨密度显著低于实验组（P<0.05）,牙槽骨丧失、IL-1、IL-1α、TGF、TNF-α、TNF-β显著高于实验组（P<0.05）。结论：雷洛昔芬可减少PMO牙周炎症小鼠牙槽骨吸收、破骨细胞因子表达,防止牙槽骨破坏而有效防止牙周炎进展。

Effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis

PURPOSE: To investigate the effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis (PMO). METHODS: No specific pathogen grade Wistar female rats purchased from Beijing Institute of Medical Device Testing were divided into 3 groups after 7 days of adaptive feeding, 15 cases in each group. Equal weight of peri-ovary fat tissue was cut in blank group; Rats in the control group received bilateral ovary resection combined with ligation with orthodontic ligation wire, while rats in experimental group received bilateral ovary resection + ligation with orthodontic ligation wire + raloxifene treatment. The activity, diet, weight and the incidence of tooth loosening were observed every day. Bone mineral density and bone resorption were measured by CT scanner, alveolar bone destruction was observed by stereomicroscope, and osteoclast related factors such as interleukin-1 (IL-1), IL-1α, transforming growth factor (TGF), tumor necrosis factor-α(TNF-α) and TNF-β were detected by enzyme-linked immunosorbent assay (ELISA). SPSS 18.0 software package was used for data analysis. RESULTS: New bone formation was the lowest in the control group, followed by experimental group and blank group. The results of methylene blue staining showed that bone resorption was significant in the control group, and bone destruction was also seen under stereomicroscope; bone resorption and bone destruction of the experimental group were better than those of the control group. Compared with the blank group, alveolar bone density decreased, alveolar bone loss, IL-1, IL-1α, TGF, TNF-α, TNF-β increased in control group and experimental group. The alveolar bone density of the control group was significantly lower than that of the experimental group (P<0.05), and alveolar bone loss, IL-1, IL-1α, TGF, TNF-α, and TNF-β were significantly higher than those of the experimental group (P<0.05). CONCLUSIONS: Raloxifene can reduce alveolar bone resorption and osteoclast cytokine expression in mice with PMO periodontal inflammation, prevent alveolar bone destruction and effectively prevent the progression of periodontitis.