| 福建省2012-2018年布鲁氏菌分离株的MLST分型 |
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| 引用本文： | 陈志平,韩腾伟,林代华,周淑姮,刘维俊,刘菁,肖方震,邓艳琴. 福建省2012-2018年布鲁氏菌分离株的MLST分型[J]. 中国人兽共患病杂志, 2020, 36(12): 982-987. DOI: 10.3969/j.issn.1002-2694.2020.00.161 |
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| 作者姓名： | 陈志平 韩腾伟 林代华 周淑姮 刘维俊 刘菁 肖方震 邓艳琴 |
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| 作者单位： | 1.福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州 350001;2.福建医科大学公共卫生学院,福州 350004 |
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| 基金项目： | 国家科技重大专项(No.2018ZX10734401-007)、福建省自然科学基金(No.2016J01347)和福建省科技创新平台建设项目(No.2019Y2001)联合资助 |
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| 摘 要： | 目的 探讨2012-2018年福建省布鲁氏菌分离株种群结构及遗传进化关系。方法 采用多位点序列分型(Multilocus sequence typing,MLST)对43 株布鲁氏菌分离株的9 个基因位点的序列进行测定,与标准序列比对后确定菌株ST型,通过Bionumerics 6.6软件对其进行聚类分析。结果 43 株布鲁氏菌分离株中,38 株为ST8型,5 株为ST17型。结论 福建省布鲁氏菌主要流行株为ST8型。MLST是研究布鲁氏菌遗传特征的重要方法。
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| 关 键 词： | 布鲁氏菌 多位点序列分型 遗传进化关系 |
| 收稿时间： | 2020-01-08 |
MLST typing of Brucella isolates in Fujian Province from 2012 to 2018 |
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| CHEN Zhi-ping,HAN Teng-wei,LIN Dai-hua,ZHOU Shu-heng,LIU Wei-jun,LIU Jing,XIAO Fang-zhen,DENG Yan-qin. MLST typing of Brucella isolates in Fujian Province from 2012 to 2018[J]. Chinese Journal of Zoonoses, 2020, 36(12): 982-987. DOI: 10.3969/j.issn.1002-2694.2020.00.161 |
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| Authors: | CHEN Zhi-ping HAN Teng-wei LIN Dai-hua ZHOU Shu-heng LIU Wei-jun LIU Jing XIAO Fang-zhen DENG Yan-qin |
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| Affiliation: | 1.Fujian Center for Disease Control and Prevention,Fujian Provincial Key Laboratory for Zoonosis Research,Fuzhou 350001,China;2.College of Public Health, Fujian Medical University, Fuzhou 350004, China |
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| Abstract: | To investigate the population structure and genetic evolution of Brucella isolates in Fujian Province from 2012 to 2018, strains were genotyped by multilocus sequence typing (MLST). The allele types of 9 gene loci in 43 Brucella isolates were defined after comparison with the standard sequence, and the ST types of the strains was determined according to the allele types of the nine loci. Bionumerics6.6 software was used for cluster analysis. Of the 43 strains of Brucella isolates, 38 were ST8 type, and 5 were of ST17 type. The MLST typing results of Brucella isolates in Fujian Province indicated that the main circulating strain in this area was ST8 type. MLST is an important method for studying the genetic characteristics of Brucella. |
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| Keywords: | Brucella Multilocus sequence typing genetic evolution relationship |
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