长链非编码RNA PCBP1-AS1在乳腺癌组织中的表达及临床意义

目的:研究多聚胞嘧啶结合蛋白1反义长链非编码RNA（lncRNA PCBP1-AS1）在乳腺癌组织中的表达及临床意义。方法:选取2013年5月至2016年4月在本院进行手术治疗的105例乳腺癌患者作为研究对象，将切除的癌组织作为试验组，同时取同一患者的癌旁（距肿瘤边缘＞2 cm）组织作为对照组。用实时荧光定量PCR（qRT-PCR）检测lncRNA PCBP1-AS1表达情况；分析lncRNA PCBP1-AS1与乳腺癌病理参数关系；分析lncRNA PCBP1-AS1表达情况对乳腺癌患者生存情况的影响；Cox回归分析影响乳腺癌的预后因素。结果:试验组lncRNA PCBP1-AS1表达明显低于对照组（P<0.05）；乳腺癌患者癌组织中lncRNA PCBP1-AS1表达水平与患者年龄、肿瘤直径、绝经情况和病理类型相关性不明显（P>0.05），与淋巴结转移和TNM分期有关（P<0.05）；绘制乳腺癌患者术后36个月生存曲线，lncRNA PCBP1-AS1高表达患者的总生存率明显高于低表达患者（P<0.05）；对lncRNA PCBP1-AS1表达、淋巴结转移和TNM分期进行Cox回归分析，显示lncRNA PCBP1-AS1表达是影响乳腺癌患者预后的独立保护因素，TNM分期是影响乳腺癌患者预后的独立危险因素。结论:乳腺癌患者癌组织中lncRNA PCBP1-AS1呈低表达，其表达水平与淋巴结转移和TNM分期有关，lncRNA PCBP1-AS1表达是乳腺癌预后独立保护因素，可作为乳腺癌预后判断指标之一，有望成为乳腺癌治疗的潜在靶点。

Expression and clinical significance of long non-coding RNA PCBP1-AS1 in breast cancer tissues

Objective:To study the expression and clinical significance of long non-coding RNA poly C binding protein-1 antisense ribonucleic acid 1(lncRNA PCBP1-AS1) in breast cancer.Methods:105 patients with breast cancer who underwent surgical treatment in our hospital from May 2013 to April 2016 were selected as the study subjects.The resected cancer tissues were taken as the experimental group，and the adjacent tissues(2 cm from the edge of the tumor) of the same patient were taken as the control group.The expression of lncRNA PCBP1-AS1 was detected by real-time fluorescence quantitative PCR(qRT-PCR).The relationship between lncRNA PCBP1-AS1 and pathological parameters of breast cancer was analyzed.The influence of the expression of lncRNA PCBP1-AS1 on survival of breast cancer patients was analyzed，and the prognostic factors of breast cancer were analyzed by Cox regression analysis.Results:The expression of lncRNA PCBP1-AS1 in the experimental group was significantly lower than that in the control group(P<0.05).The expression level of lncRNA PCBP1-AS1 in breast cancer tissues was not significantly correlated with age，tumor diameter，menopausal status and pathological type(P>0.05)，but with lymph node metastasis and TNM stage(P<0.05).Survival curves of breast cancer patients after 36 months were drawn.The overall survival rate of patients with high expression of lncRNA PCBP1-AS1 was significantly higher than that of patients with low expression of lncRNA PCBP1-AS1(P<0.05).Cox regression analysis of the expression of lncRNA PCBP1-AS1，lymph node metastasis and TNM stage showed that the expression of lncRNA PCBP1-AS1 was an independent protective factor for the prognosis of breast cancer patients，and TNM stage was an independent risk factor for the prognosis of breast cancer patients.Conclusion:The expression of lncRNA PCBP1-AS1 in breast cancer tissues is low，and its level is related to lymph node metastasis and TNM stage.The expression of lncRNA PCBP1-AS1 is an independent prognostic protective factor for breast cancer.It can be used as one of the prognostic indicators of breast cancer and is expected to become a potential target for breast cancer treatment.