| ANRIL对胃癌细胞增殖的影响 |
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| 引用本文： | 陈晓宇1,王正彩1,朱明翰1,成秉林2,张淑君1. ANRIL对胃癌细胞增殖的影响[J]. 现代肿瘤医学, 2020, 0(20): 3470-3473. DOI: 10.3969/j.issn.1672-4992.2020.20.002 |
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| 作者姓名： | 陈晓宇1 王正彩1 朱明翰1 成秉林2 张淑君1 |
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| 作者单位： | 1.哈尔滨医科大学附属第四医院病理科，黑龙江 哈尔滨 150001；2.哈尔滨医科大学附属第一医院中西医结合科，黑龙江 哈尔滨 150001 |
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| 基金项目： | 黑龙江省中医药管理局资助项目（编号:ZHY18-142） |
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| 摘 要： | 目的:研究在胃癌细胞系SGC-7901中，ANRIL对其增殖的影响，并探讨该作用是否与TGF-β/Smad通路相关。方法:采用小干扰RNA（small interfering RNA，siRNA）技术对SGC-7901细胞中的ANRIL基因、TGF-β1基因分别进行干预，并对ANRIL基因及TGF-β1基因进行联合干预；应用甲基噻唑基四唑（methyl thiazolyl tetrazolium，MTT）法检测各实验组中细胞增殖能力的变化；采用Western blot法检测各实验组中TGF-β1蛋白和p-Smad蛋白的表达情况。结果:胃癌细胞系SGC-7901经转染24 h、48 h后，siANRIL组OD值明显低于NC组、MOCK组及BLANK组（P＜0.05）。siANRIL＋siTGF-β1组、siTGF-β1组OD值高于NC组、MOCK组及BLANK组（P＜0.05）。siANRIL＋siTGF-β1组OD值介于siANRIL组与siTGF-β1组之间（P＜0.05）。siANRIL组TGF-β1蛋白和p-Smad蛋白的表达高于NC组、MOCK组及BLANK组（P＜0.05）。在siTGF-β1组及siANRIL＋siTGF-β1组中TGF-β1蛋白和p-Smad蛋白的表达低于NC组、MOCK组及BLANK组（P＜0.05）。siANRIL＋siTGF-β1组TGF-β1蛋白和p-Smad蛋白的表达介于siANRIL组与siTGF-β1组之间（P＜0.05）。结论:在胃癌细胞系SGC-7901中，ANRIL对其细胞增殖发挥促进作用，且ANRIL可能是通过TGF-β/Smad信号传导通路发挥促进作用的。
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| 关 键 词： | ANRIL TGF-β1 SGC-7901细胞 细胞增殖 胃癌 |
Effect of ANRIL on proliferation of gastric cancer cells |
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| Chen Xiaoyu1,Wang Zhengcai1,Zhu Minghan1,Cheng Binglin2,Zhang Shujun1. Effect of ANRIL on proliferation of gastric cancer cells[J]. Journal of Modern Oncology, 2020, 0(20): 3470-3473. DOI: 10.3969/j.issn.1672-4992.2020.20.002 |
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| Authors: | Chen Xiaoyu1 Wang Zhengcai1 Zhu Minghan1 Cheng Binglin2 Zhang Shujun1 |
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| Affiliation: | 1.Pathology Department,the Fourth Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China;2.The Combination of Chinese and Western Science,Department of Endocrinology,the First Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China. |
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| Abstract: | Objective:To study the effect of ANRIL on the proliferation of gastric cancer cell line SGC-7901,and to explore whether this effect is related to the TGF-β/Smad pathway.Methods:Small interfering RNA (siRNA) technology was used to interfere with ANRIL gene and TGF-β1 gene in SGC-7901 cells,and combined with ANRIL gene and TGF-β1 gene.Methyl thiazolyl tetrazole (MTT) assay was used to detect the changes of cell proliferation ability in each experimental group,and Western blot was used to detect the expression of TGF-β1 and p-Smad proteins in each experimental group.Results:After 24 and 48 hours of transfection,MTT results showed that the OD value of siANRIL group was significantly lower than that of NC group,MOCK group and BLANK group in SGC-7901 cell line (P＜0.05).Compared with NC group,MOCK group and BLANK group,the OD value of siANRIL+siTGF-β1 group and siTGF-β1 group augmented (P＜0.05).The OD value of siANRIL+siTGF-β1 group was between that siANRIL group and siTGF-β1 group (P＜0.05).The expressions of TGF-β1 and p-Smad protein in siANRIL group were significantly higher than those in NC group,MOCK group and BLANK group (P＜0.05).The expression of TGF-β1 and p-Smad protein in siTGF-β1 group and siANRIL+siTGF-β1 group were significantly lower than those in NC group,MOCK group and BLANK group (P＜0.05).Contrasted with NC group and BLANK group,the expressions of TGF-β1 and p-Smad protein in siANRIL+siTGF-β1 group was between that in siANRIL group and that in siTGF-β1 group (P＜0.05).Conclusion:In gastric cancer cell line SGC-7901,ANRIL promotes cell proliferation,and ANRIL may play a role through the TGF-β/Smad signal transduction pathway. |
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| Keywords: | ANRIL TGF-β1 SGC-7901 cell cell proliferation gastric cancer |
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