miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响

目的:探讨miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响及其机制。方法:qPCR检测miR-21和PDCD4在宫颈癌组织中的表达情况；双荧光素酶实验检测miR-21和PDCD4之间的调控作用；细胞集落实验检测抑制miR-21对宫颈癌细胞增殖能力的影响情况，Transwell侵袭实验检测对宫颈癌细胞侵袭能力的影响情况。结果:与癌旁正常组织相比，miR-21在宫颈癌组织中的表达上调，PDCD4在宫颈癌组织中表达下调；双荧光素酶实验检测miR-21和PDCD4之间的调控关系，miR-21可直接调控PDCD4蛋白的表达及活性情况；抑制miR-21的表达后可以抑制宫颈癌细胞的增殖能力；抑制miR-21的表达水平后，宫颈癌细胞的侵袭能力得到一定程度的抑制。结论:miR-21可以调控PDCD4的表达影响宫颈癌细胞的增殖和侵袭能力。

Effect of miR-21 on proliferation and invasion of cervical cancer cells through regulating programmed cell death 4 gene expression

Objective:To investigate the effect of miR-21 on the expression of apoptosis-related gene 4 (PD-4) on the proliferation and invasion of cervical cancer cells and its mechanism.Methods:qPCR was used to detect the expression of miR-21 and PDCD4 in cervical cancer.Dual luciferase assay detected the regulatory role of miR-21 and PDCD4.Cell colony assay detected the effect of miR-21 on the proliferation of cervical cancer cells.Transwell invasion test detected invasion ability of cervical cancer cells.Results:The expression of miR-21 was up-regulated in cervical cancer and the expression of PDCD4 was down-regulated in cervical cancer compared with adjacent normal tissues.Dual luciferase assay detected the regulatory relationship between miR-21 and PDCD4，miR-21 can directly regulate the expression of PDCD4 protein and activity.Inhibition of miR-21 expression can inhibit the proliferation of cervical cancer cells.After inhibition of miR-21 expression levels，cervical cancer cell invasion ability was inhibited to a certain extent.Conclusion:miR-21 can regulate the expression of PDCD4 in cervical cancer cell proliferation and invasion ability.