GNA对人宫颈癌细胞增殖抑制、凋亡、迁移及细胞周期分布的影响

目的:研究新藤黄酸（gambogic acid,GNA）对人宫颈癌细胞的增殖抑制、凋亡、迁移以及细胞周期分布的影响。方法:将人宫颈癌Hela细胞分为空白对照组、25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组，进行细胞培养后采用MTT法和流式细胞术检测细胞24 h、48 h和72 h时间段的增殖抑制和凋亡情况，Transwell实验检测细胞迁移情况，流式细胞仪检测细胞周期分布，Western blot检测Bcl-2、Bax、E-cadherin和NF-κB蛋白相对表达量。结果:25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组对宫颈癌细胞的抑制增殖率在不同时间段均显著高于空白对照组，增殖抑制率随着浓度和时间的增加而升高（P＜0.05）；25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组宫颈癌细胞凋亡率在各时间段均显著高于空白对照组，凋亡率随着浓度和时间的增加而升高（P＜0.05）；25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组宫颈癌细胞的细胞迁移数量相比对照组均显著减少，细胞迁移数量随着浓度的增加而减少（P＜0.05）；GNA浓度越高，处于G0/G1期的细胞比例越高，处于G2/M和S期的细胞比例越低；25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组Bcl-2、NF-κB均低于空白对照组（P＜0.05）；25 μmol/L浓度组、50 μmol/L浓度组和100 μmol/L浓度组Bax、E-cadherin表达均高于空白对照组。结论:GNA能够促进宫颈癌细胞的凋亡，抑制细胞的增殖和迁移能力，通过改变癌细胞的周期分布降低癌细胞的增长，其能力呈现浓度依赖性。

Effects of GNA on proliferation,apoptosis,migration and cell cycle distribution of human cervical cancer cells

Objective:To study the effects of gambogic acid (GNA) on proliferation inhibition,apoptosis,migration and cell cycle distribution of human cervical cancer cells.Methods:Cervical cancer Hela was divided into blank control group,concentration of 25 μmol/L group,concentration of 50 μmol/L and 100 μmol/L concentration group,blank control group.MTT method and flow cytometry detected for 24 h,48 h and 72 h period,inhibition of proliferation and apoptosis.Transwell experiment detected cell migration.Flow cytometry detected the cell cycle distribution.The relative expression levels of Bcl-2,Bax,E-cadherin and NF-κB were detected by Western blot.Results:The inhibition rate of cervical cancer cells proliferation in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group were significantly higher than that in the blank control group at different time periods,and the inhibition rate of proliferation was increased with the increase of concentration and time (P＜0.05).The apoptosis rate of cervical cancer cells in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group was significantly higher than that of the blank control group at all time periods,and the apoptosis rate was increased with the increase of concentration and time (P＜0.05).The number of cell migration of cervical cancer cells in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group was decreased significantly compared with the control group,and the number of cell migration was decreased with the increase of concentration (P＜0.05).The higher the concentration of GNA was,the higher the proportion of cells in G0/G1 phase was and the lower the proportion of cells in G2/M and S phase was.Bcl-2 and NF-κB in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group were all lower than the blank control group (P＜0.05).Bax and E-cadherin expression were higher in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group than in the blank control group.Conclusion:GNA can promote apoptosis of cervical cancer cells,inhibit cell proliferation and migration,and its capacity is concentration-dependent.