| HBV重组抗原表位抗原性的研究 |
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| 引用本文： | 潘云华,周东霞,李嘉琦,陈元鼎. HBV重组抗原表位抗原性的研究[J]. 免疫学杂志, 2007, 23(2): 163-165,171 |
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| 作者姓名： | 潘云华 周东霞 李嘉琦 陈元鼎 |
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| 作者单位： | 大理学院基础医学院医学微生物学与免疫学教研室,云南,大理,671000;中国医学科学院医学生物研究所中心实验室,昆明,650118 |
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| 摘 要： | 目的 在外源抗原表位表达载体系统中构建并表达HBV抗原表位,并对其抗原性进行研究.方法 人工合成编码HBV "a"抗原决定簇表位中24个氨基酸(S124-147)的寡核苷酸序列,克隆入外源抗原表位表达载体系统FHV-RNA2-I3位点,构建重组质粒,在原核pET系统(BL21细胞)中表达.以表达产物为包被抗原对其抗原性进行研究.结果 嵌和蛋白与抗-HBs血清可发生特异性结合反应.结论 在外源抗原表位表达系统中表达的HBV抗原表位具有良好的抗原性.
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| 关 键 词： | HBV 原表位 抗原性 FHV-RNA2载体系统 |
| 文章编号： | 1000-8861（2007）02-0163-04 |
| 修稿时间： | 2006-07-102006-10-08 |
Antigenicity of HBV recombinant epitope |
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| PAN Yun-hua,ZHOU Dong-xia,LI Jia-qi,CHEN Yuan-ding. Antigenicity of HBV recombinant epitope[J]. Immunological Journal, 2007, 23(2): 163-165,171 |
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| Authors: | PAN Yun-hua ZHOU Dong-xia LI Jia-qi CHEN Yuan-ding |
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| Affiliation: | Department of Microbiology and Immunology, School of Preclinical Medicine, Dali University, Dali 671000, China |
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| Abstract: | Objective To construct and express HBV recombinant epitope in a foreign epitope presenting vector, and to study antigenicity of HBV epitope. Methods Oligonucleotide sequences encoding 24 amino acids (aaS124-147) within 'a' antigenic determinant were synthesized and inserted into a foreign epitope-presenting carrier I3 site (FHV-RNA2-cDNA-I3). HBV epitope was expressed in a prokaryotic cellular system (BL21 cell). The expression product was analyzed and its antigenicity was further studied by ELISA and Western blot method with chimeric protein as coating antigen. Results The chimeric protein could reacted with anti-HBs sera specially. Conclusion HBV epitopes expressed in a foreign epitope-presenting carrier possess good antigenicity. |
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| Keywords: | HBV Epitope Antigenicity FHV-RNA2 carrier system |
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