[H]WIN 35,428 binding in the human brain

The binding of [³H]WIN 35,428 was studied in post-mortem human brain, including extrastriatal regions. In the putamen, dopamine almost completely inhibited the [³H]WIN 35,428 binding. Paroxetine inhibited the binding with similar affinity as cocaine, in the range 200–300 nM. In the frontal cortex, [³H]WIN 35,428 labelled cocaine- and alaproclate sensitive binding sites, of which a major fraction was of protein nature. The elucidation of the cocaine sensitive sites in the frontal cortex should be the subject of further research.