槲皮素对结直肠癌细胞SW480增殖与Bcl-2、C-myc表达的影响

目的:研究槲皮素对结直肠癌细胞SW480增殖与B细胞淋巴瘤/白血病-2(Bcl-2)、癌基因C-myc表达的影响。方法:5、10、20、40、80、160μmol/L槲皮素处理SW480细胞后,采用MTT法检测SW480细胞增殖情况,计算细胞增殖抑制率;实时荧光定量反相聚合酶链反应(qRT-PCR)和Western blot法检测Bcl-2、C-myc mRNA和蛋白表达水平。结果:槲皮素对SW480细胞有明显抑制作用,能够诱导SW480细胞凋亡,且与剂量、时间呈正相关;40μmol/L槲皮素可明显抑制Bcl-2、C-myc mRNA的表达;处理48、72 h时40μmol/L槲皮素明显抑制Bcl-2、C-myc蛋白的表达(P<0.05)。结论:槲皮素能够抑制SW480细胞增殖,且呈时间-剂量依赖性,槲皮素可能通过下调Bcl-2、C-myc的表达水平而发挥抗结直肠癌作用。

Effects of Quercetin on Cell Proliferation and Expression of Bcl-2 and C-myc in Colorectal Cancer Cell Line

OBJECTIVE： To observe the inhibitory effect of quercetin on the proliferation of colorectal cancer cell SW480 and the proliferation of Bcl-2 and C-myc. METHODS： SW480 cells were treated with quercetin at various concentrations （5, 10, 20, 40, 80 and 160μmol/L）, and then the proliferation of SW480 was detected by MTT assay, to calculate the inhibitory rate of cell proliferation. The mRNA and protein of Bcl-2 and C-myc were detected by realtime RT-PCR and Western blot, respectively. RE- SULTS： Quercetin could induce the apoptosis of SW480 cells and significantly inhibited proliferation of SW480 in a dose and time-dependent manner. 40 μmol/L quercetin could significantly inhibit the mRNA expression of Bcl-2 and C-myc; 40 μmol/L quer- cetin could significantly inhibit the protein expression of Bcl-2 and C-myc after 48 and 72 h （P〈0.05）. CONCLUSIONS： Querce- tin could inhibit the proliferation of SW480 cells in time and dose dependent manner. Quercetin could depress colorectal cancer through down-regulating the expression of Bcl-2 and C-myc.