结内非霍奇金淋巴瘤微卫星位点BAT-26和BAT-25的分析

目的：通过微卫星位点BAT-26和BAT-25的分析，探讨结内非霍奇金淋巴瘤是否存在微卫星不稳定性。方法：收集手术活检所得51例结内非霍奇金淋巴瘤和9例淋巴结反应性增生的冷冻标本，并分离基因组DNA，通过PCR法扩增微卫星位点BAT-26和BAT-25，应用全自动DNA测序仪和GeneScan3．1软件进行片段分析，观察这两个位点重复序列长度的变化。以已知遗传性非息肉病性结直肠癌的高度微卫星不稳病例10例为阳性对照。结果：扩增成功的标本均未见BAT-26或BAT-25位点单碱基重复序列大小异常。结论：采用结直肠肿瘤中高度敏感的微卫星稳定性检测位点BAT-26和BAT-25，未发现结内非霍奇金淋巴瘤存在高度微卫星不稳，是否存在低度微卫星不稳以及错配修复基因是否参与恶性淋巴瘤的发病有待进一步研究。

Microsatellite analysis of BAT-26 and BAT-25 in nodal non-Hodgkin′s lymphomas

Purpose:To investigate the existence of micro sa tellite instability in nodal non-Hodgkin's lymphomas by microsatellite analysis of BAT-26 and BAT-25.Methods:Frozen tissues of 51 nodal non-Hodgkin's lymphomas and 9 lymphoid reactive hyperplasia were collected by surgical biopsy. Genomic DNA was extracted. Microsatellite alterations of BAT-26 and BAT-25 were detected b y polymerase chain reaction(PCR)followed by fragment analysis using automatic DNA sequencer and GeneScan 3.1 software. 10 cases of hereditary nonpolyposis col orectal cancers with known high frequency microsatellite instability (MSI-H) we re retrieved as positive controls. Results:No aberration of mon onucleotide duplication in the microsatellite markers BAT-26 and BAT-25 was ob served in all successfully amplified specimens.Conclusions:The microsatellite analysis of BAT-26 and BAT-25, two highly sensitive markers to microsatellite status in colorectal tumors, demonstrated no evidence of MSI-H i n nodal non-Hodgkin's lymphomas. The existence of low frequency microsatellite instability and participation of mismatch repair genes in lymphomagenesis remain to be determined by further studies.