慢性低氧性肺动脉高压大鼠肺组织P53表达增高

目的观察P53在低氧性肺动脉高压大鼠肺组织中的表达。方法采用减压低氧方法复制大鼠慢性低氧性肺动脉高压模型;取肺组织,常规SABC免疫组化法染色和Hoechst染色,观察P53表达的变化及细胞凋亡变化。结果P53在慢性肺动脉高压大鼠肺小动脉壁中有少量表达,其中低氧3周组>低氧2周组>正常组。Hoechst染色显示肺组织凋亡细胞增加,其变化趋势与P53的表达变化趋势相同。结论在慢性低氧性肺动脉高压形成过程中,肺小动脉壁发生了增殖和凋亡,程度随低氧时间的延长而增加,同时P53表达增多,提示P53参与了慢性低氧性肺动脉高压肺小血管重建的调控。

Increased P53 expression in the lung tissue of rats with chronic hypoxic pulmonary hypertension

Objective To delinate the expression of P53 in the lung tissue of rats with chronic hypoxic pulmonary hypertension(HPH).Mehtods The chronic HPH rat models were duplicated by low-pressure and hypoxic approaches.The expression of P53 in the lung tissue of HPH rats was explored by ABC immunohistochemistry(IHC).The number of apoptosis cells were measured by Hoechst staining.Results IHC staining showed that the positive cell of P53 in lung tissue was increased in hypoxic group,especially cells in the wall of pulmonary arteriolar,and the expression intensity of P53 in hypoxic> hypoxic 2 weeks group>control group.Hoechst staining showed that the number of apoptosis cell in the lung tissue was also increased.The number of apoptosis cells in hypoxic was significantly more than those in 2 weeks hypoxic group and control group.Conclusions In the process of chronic HPH rats,both the proliferation and aopotosis in the wall of pulmonary arteriolar are increased,and the expression of P53 in the lung tissue has an increasing tendency.The activity of P53 may play a regulation role in pulmonary arteriolar reconstruction in chronic HPH rats.