复脉汤对心肌缺血大鼠心律失常发生率及Cx43蛋白表达影响的研究

目的观察复脉汤灌胃预处理对心肌缺血大鼠心律失常发生率及Cx43表达的影响,探讨其发生机制。方法采用结扎冠状动脉左前降支的方法制备SD雄性大鼠的心肌缺血模型,冠脉结扎1h后观察复脉汤灌胃预处理对大鼠体表心电图及心律失常评分的影响;蛋白免疫印迹法（Western blot）检测缝隙连接蛋白43（Cx43）总量表达的变化。结果 1心律失常评价：急性缺血期间,各组大鼠均出现不同程度的心律失常,模型组心律失常发生率为87.5%,复脉汤低、中、高剂量组心律失常发生率分别62.5%,50.0%,62.5%,心律失常评分比较,3组均低于模型组（P〈0.05）。2Cx43蛋白的表达：Cx43相对表达量（Cx43/GAPDH）组间比较,假手术组显著高于模型组（P〈0.01）;复脉汤高剂量组显著高于模型组（P〈0.01）,作用与酒石酸美托洛尔相似;复脉汤低、中剂量组高于模型组（P〈0.05）,复脉汤低、中、高剂量组组间比较无统计学意义（P〉0.05）。结论复脉汤灌胃预处理可以降低冠脉结扎1h后心肌缺血大鼠的心律失常发生率,提高Cx43/GAPDH,其机制可能为改善心肌组织的供血和供氧而提高Cx43的表达,降低心梗并发症心律失常的发生。

Study the Effect of Fumai Decoction on the Incidence of Arrhythmia in Rat Myocardial Ischemia Model and the Expression of Cx43

Objective To investigate the effect and possible mechanism of pretreatment of Fumai decoction on the incidence of arrhythmia in rat myocardial ischemia model and the expression of Cx43. Methods SD male rats of myocardial ischemia model were induced by left anterior descending ligation. The continuous electrocardiographic was monitored for one hour to observe the effect of pretreatment of Fumai decoction and the arrhythmia score. The Cx43 expression was characterized by Westernblot. Results（1）Evaluation of arrhythmia：after myocardial ischemia models were induced,all the groups responded in arrhythmia,but with different degrees. The incidence of arrhythmia in model group was 87. 5%. The incidence of arrhythmia in low dose,middle dose and high dose groups of Fumai decoction were 62.5%,50.0%,62.5%,respectively. Compared the arrhythmia score,all the three groups of Fumai decoction was lower than the model group（P〈0.05）.（2）Expression of Cx43：ham operation group showed the highest relative expression of Cx43/GAPDH and higher than the model group（P〈0.01）. High dose groups of Fumai decoction was significantly higher than model group（P〈0.01）,which was similar to tartaric acid metoprolol group. Low dose and middle dose of Fumai decoction were higher than model group（P〈0.05）,while the three groups of Fumai decotion showed no statistical difference（P〈0.05）.Conclusion Pretreatment of Fumai decoction can reduce the incidence of arrhythmia in myocardial ischemia model induced by left anterior descending ligation and increase the relative expression of Cx43/GAPDH. This might be the result of improving the myocardial tissue blood flow and oxygen supply to improve the expression of Cx43,reduce the occurrence of arrhythmia.