| HPLC测定滇紫草及其两个近缘种中乙酰紫草素的含量 |
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| 引用本文： | 张超,何宝丰,陈世友,张庆芝. HPLC测定滇紫草及其两个近缘种中乙酰紫草素的含量[J]. 云南中医学院学报, 2014, 0(6): 17-19,22 |
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| 作者姓名： | 张超 何宝丰 陈世友 张庆芝 |
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| 作者单位： | [1]铜梁县中医院,重庆铜梁402560; [2]云南中医学院,云南昆明650500 |
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| 摘 要： | 目的建立滇紫草、密花滇紫草和昆明滇紫草中乙酰紫草素的含量测定方法。方法采用HPLC法测定滇紫草、密花滇紫草和昆明滇紫草中乙酰紫草素的含量。结果乙酰紫草素在0.0952~1.9030μg范围内呈良好的线性关系。精密度、稳定性、重复性和加样回收率均达到要求。结论该方法简便、准确、重现性好,可用于滇紫草及其两个近缘种中乙酰紫草素含量的测定。
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| 关 键 词： | HPLC 滇紫草 乙酰紫草素 |
Content Determination of Acetylshikonin in Onosma Paniculatumand Its Two Closely Related Species by HPLC |
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| ZHANG Chao,HE Bao-feng,CHEN Shi-you,ZHANG Qing-zhi. Content Determination of Acetylshikonin in Onosma Paniculatumand Its Two Closely Related Species by HPLC[J]. Journal of Yunnan College of Traditional Chinese Medicine, 2014, 0(6): 17-19,22 |
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| Authors: | ZHANG Chao HE Bao-feng CHEN Shi-you ZHANG Qing-zhi |
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| Affiliation: | ZHANG Chao, HE Bao-feng, CHEN Shi-you, ZHANG Qing-zhi( 1. TCM Hospital of Tongliang, Tongliang, Chongqing 402560, China; 2. Yunnan University of TCM, Kunming 650200, China) |
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| Abstract: | Objective To establish the determination method of acetylshikonin in three species,Onosma paniculatum Bur. et Fr.,O. cingulatum W. W. smith et J. E. Jeffrey and O. confertum W. W. Smith.. Methods Using HPLC as quantitative analysis means and acetylshikonin as the detection of targets,the quality standards of above three species were studied. Results The acetylshikonin had good linear relationship in the range of 0.0952 ~1.9030μg. Precision,stability,repeatability and recovery are achieved. Conclusion The method is simple,accurate,reproducible and suitable for determining the acetylshikonin in Onosma paniculatum and its two closely related species. |
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| Keywords: | HPLC Onosma paniculatum Bur.et Fr. acetylshikonin |
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