| 白纹伊蚊防御素基因的体外扩增及鉴定 |
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| 引用本文： | 支国舟,陈晓光,吴焜. 白纹伊蚊防御素基因的体外扩增及鉴定[J]. 中国热带医学, 2003, 3(3): 285-286 |
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| 作者姓名： | 支国舟 陈晓光 吴焜 |
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| 作者单位： | 第一军医大学寄生虫学教研室,广州市,510515 |
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| 基金项目： | 广州市科技计划项目(2001-J-001-01) |
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| 摘 要： | 目的 克隆白纹伊蚊的防御素基因并对其进行序列分析。方法 提取白纹伊蚊基因组DNA，根据埃及伊蚊防御素基因序列设计合成引物，PCR产物克隆到T载体中进行测序和分析。结果 从白纹伊蚊基因组DNA中扩增出了约375bP的片段，PCR产物经测序后与已发表的埃及伊蚊防御素基因比较，具有很高的同源性。结论 克隆了白纹伊蚊的防御素基因，为下一步蚊虫防御素蛋白的研究莫定了基础。
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| 关 键 词： | 白纹伊蚊 防御素 基因 体外扩增 鉴定 |
| 文章编号： | 1009-9727(2003)03-0285-02 |
| 修稿时间： | 2002-12-30 |
Cloning and identifying of the defensin gene of Aedes albopictus |
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| ZHI Guo-zhou,CHEN Xiao-guang,WU Kun. Cloning and identifying of the defensin gene of Aedes albopictus[J]. China Tropical Medicine, 2003, 3(3): 285-286 |
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| Authors: | ZHI Guo-zhou CHEN Xiao-guang WU Kun |
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| Affiliation: | ZHI Guo-zhou,CHEN Xiao-guang,WU Kun.Department of Parasitology,The First Military Medical University,GuangZhou 510515,Guangdong,P.R.China. |
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| Abstract: | Objective To clone the defensin genes from Aedes albopictus. Methods The genomic DNA were extracted from Aedes aegypti.The PCR was carried out and the PCR products were purified and cloned into plasmid T-vector.The recombinant plasmid was sequenced. Results The amplified products from A.albopictus were about 375bp,The sequence analysis showed that the amplified fragment from A.albopictus had a high homology compared with the published defensin sequence from A.aegypti. Conclusion The defensin gene of A.albopictus have been cloned. |
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| Keywords: | Mosquito Defensin Gene Clone PCR. |
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