新疆阿魏菇提取物对4种肿瘤细胞p53表达的影响

目的：研究新疆阿魏菇提取物影响体外培养的肿瘤细胞p53表达，从细胞凋亡角度探讨其抗肿瘤机制。方法：采用肿瘤细胞体外培养技术及流式细胞技术．观察不同剂量的阿魏菇水提物及醇提物对体外培养的4种类型肿瘤细胞p53表达的影响。结果：受试物对4种类型肿瘤细胞均具有较强的杀伤作用．尤其以0．1g／ml剂量组为明显；经受试物处理后，4种类型肿瘤细胞均观察到细胞核固缩，DNA浓缩并向核膜靠拢形成浓染致密颗粒．并有典型凋亡小体出现。提示．受试物可能通过诱导肿瘤细胞凋亡而发挥抗肿瘤作用；流式细胞仪检测结果显示，受试物处理后，4种类型肿瘤细胞p53表达水平均有不同程度的上调，尤其以醇提物作用24h后影响Q3细胞p53表达水平最为明显。结论：阿魏菇提取物中的各组分可协同作用诱导肿瘤细胞促凋亡基因的表达，从而达到抗肿瘤之目的。

Experimental study on the antitumor mechanism of pleurotes sapidus in vitro

Objective: The antitumor mechanism of Pleurotes sapidus on 4 kinds of tumor cell lines (Q3, MGC 803, Hela, SPC A 1) was studied. Methods: Four tumor cell lines were cultured in vitro. We investigated the expression of p53 by FACS techniques, after treated by water extract or ethanol extract from Pleurotas sapidus with doses 10 1 ~10 3 g/mL. Results: Experimental study of MTT and HT were used to determine the antitumor effects of Pleurotas sapidus from inducing apoptosis of cancer cells. The results found that the incidence rates of apoptosis were remarkably increased after treating 12 h and 24 h by ethanol extractive (0.1 g/ml) and water extractive (0.1 g/ml). The effects of ethanol extractive and water extractive on expression of p53 gene related to apoptosis were examined by FACS technique, the results showed that the expression of p53 protein in the groups of ethanol extractive and water extractive after treating 12 hr and 24 hr were significantly increased. Conclusion: The antitumor effect of Pleurotas sapidus is evident, and its mechanisms are probably involved in the regulation of apoptosis by inducing the gene expressions related to apoptosis, and up adjustment improving apoptosis gene expression (p53).