Expression and evaluation of Chikungunya virus E1 and E2 envelope proteins for serodiagnosis of Chikungunya virus infection |
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Authors: | Cho Byungki Jeon Bo-Young Kim Jungho Noh Jaesang Kim Jiha Park Minjung Park Sun |
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Institution: | 1Standard Diagnostics Inc., Yongin, Korea.;2Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.;3Department of Microbiology and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. |
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Abstract: | PurposeChikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens.Materials and MethodsCHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA.ResultsThe recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%.ConclusionThe recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection. |
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Keywords: | Chikungunya virus E1 and E2 envelope protein baculovirus indirect IgM ELISA |
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